Application note

Quantitation of low Tryptophan and Tyrosine peptides using the Fluidity One

While UV absorbance presents a quick and simple method for the quantitation of proteins and peptides, the sequence must contain Trp or Tyr residues for detection at 280 nm. Although the A205 method presents an alternative, this has its own limitations in terms of buffer absorbance.

  • This Application note shows that MDS analysis offers a compelling alternative to A205, providing quantification at higher accuracy and precision, as well as providing size measurement in the same test.
  • It was also found that there was lower variation across repeat measurements in the MDS Fluidity One data. 
  • This enables researchers to rapidly and with little sample loss have confidence in both the concentration and quality of samples prior to subsequent downstream analysis.

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