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Fast calculations of hydrodynamic radius from structural models that are useful aides in interpreting microfluidic diffusional sizing measurements.
Hullrad was developed by PJ Fleming and KG Fleming; you can find their original portal, which provides more comprehensive parameter prediction, here: Hullrad
We have adapted their code to focus on hydrodynamic radius calculations that are useful aides in interpreting the microfluidic diffusional sizing measurements obtained on Fluidic Analytics instruments. We have also added a convenient front end to make it easier to work directly with pdb files from the RCSB. The underlying calculations are unmodified from the original code except for one modification to allow the calculation of hydrodynamic radius for structures that contain non-glycine residues lacking beta carbon atoms. If your structure of interest contains these, they will be modeled as glycine residues and a list of the modified residues will be provided on-screen with the results modified from the original code.
If you use Hullrad in your work please cite:
P. J. Fleming & K. G. Fleming. HullRad: Fast Calculations of Folded and Disordered Protein and Nucleic Acid Hydrodynamic Properties. Biophys J., 2018, 114, 856-869. DOI:10.1016/j.bpj.2018.01.002
The following non-glycine residues were incomplete in the structure and so have been renamed to glycine:
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