Publication

Putative interaction site for membrane phospholipids controls activation of TRPA1 channel at physiological membrane potentials.

Lucie Macikova, Viktor Sinica, Anna Kadkova, Sandrine Villette, Alexandre Ciaccafava, Jonathan Faherty, Sophie Lecomte, Isabel D. Alves, Viktorie Vlachova.

The FEBS Journal 14931, 2019 May 20

Macikova et al use Microfluidic Diffusional Sizing (MDS) along with other techniques to understand the role phosphatidylinositol-4,5-bisphosphate (PIP2) has in the regulation of transient receptor potential ankyrin 1 (TRPA1) channel. The Fluidity One was used to create binding curves of the interactions between two specific peptides (L992-N1008 and T1003-P1034) and model lipid membranes in the presence of PIP2.

The paper demonstrates that the two peptides (L992-N1008 and T1003-P1034) interact with lipid membranes only if PIP2 is present and their affinities depend on the presence of calcium. The paper also shows tht the putative phosphoinositide-interacting domain contributes to the stabilization of the TRPA1 channel gate.

binding curves from macikova et al 2019

Fig 1. shows the binding curves obtained using the Fluidity One for each of the two peptides binding to the lipid membrane in the presence of PIP2.

Find the paper here    Poster Video Here

  • Publications and resources

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    • Posters10 September 2019

      Fluidity One-W protein interactions poster

      Poster summarising the work done by our internal applications team using MDS on protein interactions with proteins, lipids and antibodies as well as other published work using MDS to analyse protein interactions.

    • Publication25 July 2019

      Microfluidic diffusional sizing (MDS) in the literature

      Document referencing all the publications where microfluidic diffusional sizing (MDS) has been used.

    • Video24 July 2019

      Lucie Machikova - EBSA IUPAP 2019 Poster video

      Lucie Machikova talking through her poster detailing the work carried out in her publication "Putative interaction site for membrane phospholipids controls activation of TRPA1 channel at physiological membrane potentials".

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