The molecular weight of proteins is an important analytical parameter, but it does not provide information on the crucial secondary, tertiary or quaternary structural components which are often vital for their proper function.
By observing the size in solution as well as molecular weight, we can gain insights into the conformation of proteins and their complexes.
Here we observe the relationship between Mw and Rh (as measured by microfluidic diffusional sizing, MDS) in a range of proteins, and note how this relationship can indicate structure.
- The use of MDS in elucidating the Mw/Rh relationship in proteins where shape is a critical aspect of function provides a rapid yet insightful method for protein scientists.
- By observing the Rh to Mw relationship, we can elicit structural clues that can highlight clear differences between monomeric globular proteins and those that assume other conformations.