Protein labelling procedure using Alexa Fluor 488 NHS ester

Long form protein labelling protocol using Alexa Fluor 488 NHS Ester from Thermo Fisher Scientific. Includes troubleshooting, purification steps and an example calculation to help with concentration and volume calculations. Procedure designed to be used in conjunction with Fluidity One-W where free unbound dye would effect readings however this procedure can be used to label proteins to be used in other applications.

If you need a hand with concentration and volume calculations for labelling proteins head over here for our labelling calculator:

Labelling Calculator

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