Microfluidic diffusional sizing (MDS) in the literature

Publication01 August 2019

Analysis of αB-crystallin polydispersity in solution through native microfluidic electrophoresis

In this paper, the authors utilise microfluidic diffusional sizing and electrophoresis to characterise the oligomeric states of the chaperone protein alphaB-crystallin.

Publication29 July 2019

On-chip label-free protein analysis with downstream electrodes for direct removal of electrolysis products

Saar et al designed and demonstrated a single step lithography approach to fabricate microfluidic devices that can be used to apply high electric fields without the formation of any electrolysis products in the electrophoresis area.

Publication25 July 2019

Gradient-free determination of isoelectric points of proteins on chip

Lapinska et al. using a microfluidic system built in house design a new technique to determine a protein's isoelectric point (pI) based on microfluidic free-flow electrophoresis (μFFE). The approach exploits temporal rather than spatial pH gradients. To demonstrate the effectiveness of this method the pI of 7 different proteins of known pI were tested; β-lactoglobulin, ribonuclease A, ovalbumin, human transferrin, ubiquitin and myoglobin. The paper shows that this method is successful in determining the pI using this new technique without the requirement of generating and maintaining pH gradients which is often challenging for other techniques. The technique requires low voltages and low sample consumption. The paper also shows that using this technique it is possible to estimate the pI values for a wide range of proteins measuring at only two pH values, suggesting that this technique is rapid and accurate on small volume samples.

Publication24 July 2019

Putative interaction site for membrane phospholipids controls activation of TRPA1 channel at physiological membrane potentials.

Macikova et al use Microfluidic Diffusional Sizing (MDS) along with other techniques to understand the role phosphatidylinositol-4,5-bisphosphate (PIP2) has in the regulation of transient receptor potential ankyrin 1 (TRPA1) channel. The Fluidity One was used to create binding curves of the interactions between two specific peptides (L992-N1008 and T1003-P1034) and model lipid membranes in the presence of PIP2.

Publication04 June 2019

Secondary nucleation and elongation occur at different sites on Alzheimer’s amyloid-β aggregates

In this paper MDS was used to identify two molecular chaperones which suppress secondary nucleation processes and inhibit the elongation of Aβ fibrils seen in Alzheimer's disease.

Publication06 March 2019

α-Synuclein-derived lipoparticles in the study of α-Synuclein amyloid fibril formation

This paper probes the interactions of α-synuclein with lipid particles using the Fluidity One and various other methods, to investigate the link between these interactions and α-synuclein aggregation.

Publication15 June 2018

Cooperative Assembly of Hsp70 Subdomain Clusters

Wright et al use Microfluidic Diffusional Sizing to probe the oligomerisation of the SBD641 substrate of human Hsp70. The Fluidity One was employed to verify if the fluorescent label used had an affect on measured hydrodynamic radius during the tests.

Publication02 February 2018

A microfluidic diffusion platform for characterizing the size of lipid vesicles and the thermodynamics of protein-lipid interactions

Hongze Gang in the Tuomas Knowles lab demonstrates the use of microfluidic diffusional sizing for characterizing the size of lipid vesicles and the thermodynamics of protein-lipid interactions.

Publication01 December 2017

A Microfluidic Platform for Real-Time Detection and Quantification of Protein-Ligand Interactions

In this paper, Herling et al demonstrate a microfluidic platform for the detection of protein-ligand interactions with an assay time on the second timescale and without the requirement for immobilization or the presence of a highly viscous matrix.

Publication01 December 2017

Protein Aggregate-Ligand Binding Assays Based on Microfluidic Diffusional Separation

Zhang et al again demonstrate the utility of Microfluidic Diffusional Sizing to monitor Nanobody binding to ɑ-synuclein, but this time also use MDS to monitor ɑ-synuclein fibril formation.

Publication01 December 2017

Microfluidic diffusion viscometer for rapid analysis of complex solutions.

Arosio et al demonstrate the utility of a diffusion viscometer, showing that by including tracer particles and tracking mass transport, the viscosity of complex solutions can be determined using only low quantities of sample material, with a short analysis time and can be integrated into other microfluidic elements on a chip.

Publication01 December 2017

Latent analysis of unmodified biomolecules and their complexes in solution with attomole detection sensitivity

In this paper, Yates et al show that the technology behind Fluidic Analytics instruments - microfludic diffusional sizing - can be used to accurately size a range of proteins.

Publication30 November 2017

Microfluidic diffusion analysis of the sizes and interactions of proteins under native solution conditions.

In this paper from Arosio et al microfluidic diffusional sizing (MDS) is used in combination with pre-labelled fluorescent biomolecules to size and quantify proteins in complex mixtures. Initially, MDS is compared to Dynamic Light Scatter (DLS) - and a good correlation is observed for monodispersed solutions.