Emma V. Yates, Thomas Müller, Luke Rajah, Erwin J. De Genst, Paolo Arosio, Sara Linse, Michele Vendruscolo, Christopher M. Dobson, Tuomas P.J. Knowles.
Nature Chemistry 7(10):802-9, 2015.
In this paper, Yates et al show that the technology behind Fluidic Analytics instruments—microfluidic diffusional sizing—can be used to accurately size a range of proteins. Results were compared to those obtained using Pulsed Field Gradient NMR and Analytical Ultracentrifugation and found to agree closely (Figure 1).
Finally, they demonstrate that Microfluidic Diffusional Sizing can be used to characterise protein interactions without the use of tags, using the platform to explore the binding of α-synuclein to a nanobody, NbSyn1 (Figure 2).