The KD cheat sheet

The KD cheat sheet

Protein interactions can be central to protein function and activity, so understanding the nature of these interactions is important.

KD, the dissociation constant, is how the strength of molecular interactions are defined.

This printable sheet is a handy overview of KD, answering key questions like;

  • What is KD?
  • How to calculate KD?
  • How to find KD experimentally?
  • What factors affect KD?

Download the pdf here.

  • Publications and resources

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    • Video25 July 2019

      KD video series

      Here is our series of videos that explain what te dissociation constant, KD, is and how to derrive it on a binding curve.

    • Blog03 July 2019

      Affinity vs Avidity- What's the difference?

      In this blog post we will be delving into the differences between affinity and avidity

    • Application note03 April 2019

      Determination of KD of aptamer protein interactions by microfluidic diffusional sizing

      Here the Fluidity One-W is used to experimentally determine the KD of two protein aptamer interactions, with results in good agreement with literature values. Importantly the technique required no calibration, complex setup or specialist sample preparation. Furthermore, the interaction is assessed without immobilising or altering either molecule beyond the addition of a standard fluorescent label.

    • Blog06 February 2019

      Born Slippy; Protein-Lipid interactions around the human body

      There are many proteins which interact with lipids in vivo, in complex relationships that have crucial impacts on organism health. These are often thought of as disease causing, but can also play roles in normal biochemical functions. Here are some examples of protein-lipid interactions from around the human body, which play a part in healthy activity in cells.

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