User Manuals

Fluidity One-W User manual — software version 3.8.7

Published on June 24th, 2021

The User manual for the Fluidity One-W and Fluidity One-Serum. This is for software version 3.8.7.

The Fluidity One-W and Fluidity One-Serum uses Microfluidic Diffusional Sizing (MDS) to size labeled species, such as proteins, in solution in less than 15 minutes. Titrating a binding partner against the labelled species can be used to determine binding affinity, giving a KD value as well as the size (Rh) of the labelled species and the complex.

This can be used in a laboratory environment for analysis of protein interactions, stochiometry, protein conformation, and aggregation. It can even perform analysis on proteins in complex biological backgrounds, such as cell lysates.