User Manuals

Fluidity One-W User manual — software version 3.8.0

Published on February 28th, 2020

The User manual for the Fluidity One-W. This is for software version 3.8.0.

The Fluidity One-W uses Microfluidic Diffusional Sizing (MDS) to size labeled species, such as proteins, in solution in less than 15 minutes. Titrating a binding partner against the labeled species can be used to determine binding affinity, giving a KD value as well as the size (Rh) of the labeled species and the complex.

This can be used in a laboratory environment for analysis of protein interactions, stochiometry, protein conformation, and aggregation. It can even perform analysis on proteins in complex biological backgrounds, such as cell lysates.