Fluidity One-W protein interactions poster

A poster looking at the binding affinity work that has been conducted using microfluidic diffusional sizing (MDS) both in the Fluidic Analytics applications lab as well as other published works. The poster looks at how binding affinity can be determined using MDS, protein-protein interactions as well as protein-lipid interactions. 

Read the poster for yourself to learn more! 

You can also explore our range of application notes that show the full studies mentioned in this poster as well as other applications of MDS.

Application notes

  • Publications and resources

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    • Video10 September 2019

      How does the Fluidity One-W work?

      The Fluidity One-W uses microfluidic diffusional sizing (MDS) technology to determine the binding affinity between proteins and their binding partners; whether they are aptamers, lipids, DNA, small molecules or other proteins. Watch the video to understand how the Fluidity One-W works.

    • Publication24 July 2019

      Putative interaction site for membrane phospholipids controls activation of TRPA1 channel at physiological membrane potentials.

      Macikova et al use Microfluidic Diffusional Sizing (MDS) along with other techniques to understand the role phosphatidylinositol-4,5-bisphosphate (PIP2) has in the regulation of transient receptor potential ankyrin 1 (TRPA1) channel. The Fluidity One was used to create binding curves of the interactions between two specific peptides (L992-N1008 and T1003-P1034) and model lipid membranes in the presence of PIP2.

    • Application note03 April 2019

      Determination of KD of aptamer protein interactions by microfluidic diffusional sizing

      Here the Fluidity One-W is used to experimentally determine the KD of two protein aptamer interactions, with results in good agreement with literature values. Importantly the technique required no calibration, complex setup or specialist sample preparation. Furthermore, the interaction is assessed without immobilising or altering either molecule beyond the addition of a standard fluorescent label.

Could you use MDS in your research too?

Study protein-protein, protein-DNA and protein-lipid interactions in biological mixtures with the Fluidity One-W.

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