Application noteRapid quality control of commercially available antibodies by MDS for acceptance testing
Size presents a simple metric to assess quality as an indicator of aggregation and degradation. Here MDS on the Fluidity One and SEC analysis are both used to check 6 commercially available antibodies. Both techniques sucessfully identify problem samples, but MDS does so with 80% less time and 90% less sample.
BlogProtein analysis techniques - a history and timeline
Proteins were discovered in the late 18th century, but analytical methods to observe them were not developed until more than 100 years later. This timeline shows when different techniques were developed.
BlogProtein aggregation - why it matters, and how to study it
A growing field of research is dedicated to protein aggregation, which can be useful, functional or unwanted. This article explores some of the reasons why proteins aggregate, and details the techniques available to study it.
Application noteQuantitation of low Tryptophan and Tyrosine peptides using the Fluidity One
UV absorbance is commonly used as a fast method for protein and peptide quantitation, however samples must contain tryptophan (Trp) or tyrosine (Tyr) residues to be detected. Here we demonstrate the ability of the Fluidity One to assess the concentration of a range of samples which have low or no Trp and Tyr content.
BlogHow does Microfluidic Diffusional Sizing (MDS) compare to Dynamic Light Scattering (DLS) for protein size tests?
Measuring the size of proteins provides insights into folding and conformations, aggregation and oligomerization. We compare and contrast the benefits and limitations of two popular techniques - DLS Dynamic Light Scattering, and MDS Microfluidic Diffusional Sizing.
Application noteA comparison of Microfluidic Diffusional Sizing with Dynamic Light Scattering and Taylor Dispersion Analysis
The established technologies of Dynamic Light Scattering (DLS) and Taylor Dispersion Analysis (TDA) are compared to Microfluidic Diffusional Sizing (MDS) for sizing proteins of varying molecular weights and at varying concentrations. We show that MDS offers comparable sizing of proteins over a range of sizes, and can provide consistent sizing to lower concentrations than the other techniques.
BlogHow does the Fluidity One compare to UV-Vis spectrophotometers in testing the concentration of proteins?
UV-vis absorbance testing is a popular method for rapid testing of protein concentration with only a small sample volume needed. Here we compare this method to Microfluidic Diffusional Sizing (MDS), as used on the Fluidity One, to see how the workflow, accuracy, test method, range and limitations compare.
BlogProtein size - how do I measure it, and why is it important?
An overview of why protein size matters, and what structural and functional information protein size can reveal. To understand proteins and their function, we have to understand the way they fold, aggregate and interact. Conformation is key to protein function and can be revealed by measuring size. Different methods for measuring protein size are summarised, and comparison is made, considering the method, range, cost and limitations of each technology.
BlogWhat analytical laboratory methods are available for protein quantification? How do they compare?
Quantification is an essential part of protein analysis and quality control. Here we look at the various approaches for protein quantification, their advantages, and their limitations.