Blog23 August 2019Looking at the numbers: Why protein stoichiometry matters
Accurate determination of stoichiometry for a protein complex is critical to our understanding how the complex will react to other proteins or small molecules. Here we look at how stoichiometry not only effects important viral protein-antibody interactions but also disease pathology.
Blog04 July 2019The Protein Society Symposium 2019 roundup
In this blog post we showcase videos of authors taking us through their poster presentations and highlight key papers from the talks from our favourite seven topics.
Publication04 June 2019Secondary nucleation and elongation occur at different sites on Alzheimer’s amyloid-β aggregates
In this paper MDS was used to identify two molecular chaperones which suppress secondary nucleation processes and inhibit the elongation of Aβ fibrils seen in Alzheimer's disease.
Application note04 April 2019Rapid quality control of commercially available antibodies by MDS for acceptance testing
Size presents a simple metric to assess quality as an indicator of aggregation and degradation. Here MDS on the Fluidity One and SEC analysis are both used to check 6 commercially available antibodies. Both techniques sucessfully identify problem samples, but MDS does so with 80% less time and 90% less sample.
Infographic15 March 2019The cost of poor quality protein
This infographic explores what is meant by "protein quality" - and where it can cause problems in research.
Blog08 March 2019Protein analysis techniques - a history and timeline
Proteins were discovered in the late 18th century, but analytical methods to observe them were not developed until more than 100 years later. This timeline shows when different techniques were developed.
Blog12 February 2019Protein DLS result not as expected? Try these troubleshooting tips
If your DLS result for a protein sample is not what you expected, it could be down to one of these common reasons.
Use this list to troubleshoot your DLS protein results fast.
Application note31 October 2018Protein Size as an Indication of Structure
Molecular weight (Mw) is a commonly used, and for many scientists a readily understood, parameter to describe the size of a protein or complex. Here we show how hydrodynamic radius (Rh) can be used in combination with Mw to provide insights into the shape and structure of proteins and illustrate how Mw alone may not always provide a complete picture.
Application note22 August 2018Oligomerization of Interleukin-2
A commercially available human interleukin-2 is assessed by microfluidic diffusional sizing on the Fluidity One across a dilution series. The hydrodynamic radius is observed to increase with increasing concentration, in a way which suggests a monomer-trimer equilibrium with positive cooperativity is established.
Application note22 August 2018Interleukin-2 stability in changing buffer and temperature conditions
The stability of interleukin-2 in different buffers and storage temperatures is evaluated using the Fluidity One. We find that IL-2 forms aggregates within 24 hours in some buffers, and that the Fluidity One provides a simple means to evaluate the stability of proteins across different conditions.
Blog21 June 2018How does Microfluidic Diffusional Sizing (MDS) compare to Dynamic Light Scattering (DLS) for protein size tests?
Measuring the size of proteins provides insights into folding and conformations, aggregation and oligomerization. We compare and contrast the benefits and limitations of two popular techniques - DLS Dynamic Light Scattering, and MDS Microfluidic Diffusional Sizing.
Application note14 June 2018A comparison of Microfluidic Diffusional Sizing with Dynamic Light Scattering and Taylor Dispersion Analysis
The established technologies of Dynamic Light Scattering (DLS) and Taylor Dispersion Analysis (TDA) are compared to Microfluidic Diffusional Sizing (MDS) for sizing proteins of varying molecular weights and at varying concentrations. We show that MDS offers comparable sizing of proteins over a range of sizes, and can provide consistent sizing to lower concentrations than the other techniques.
Blog17 May 2018Protein size - how do I measure it, and why is it important?
An overview of why protein size matters, and what structural and functional information protein size can reveal. To understand proteins and their function, we have to understand the way they fold, aggregate and interact. Conformation is key to protein function and can be revealed by measuring size.
Different methods for measuring protein size are summarised, and comparison is made, considering the method, range, cost and limitations of each technology.
Application note10 May 2018Detecting insulin oligomerization using microfluidic diffusional sizing
Insulin monomers self-assemble into hexamers, which is known to affect its level of uptake in the human body. Here we show that Microfluidic Diffusional Sizing (MDS) can be used to detect these changes.