Video12 August 2019Measuring IL2 Stability in different buffers using Microfluidic Diffusional Sizing (MDS)
In this video we look at how a commercially available human interleukin-2 is assessed by microfluidic diffusional sizing on the Fluidity One across a dilution series. The hydrodynamic radius is observed to increase with increasing concentration, in a way which suggests a monomer-trimer equilibrium with positive cooperativity is established.
Publication25 July 2019Gradient-free determination of isoelectric points of proteins on chip
Lapinska et al. using a microfluidic system built in house design a new technique to determine a protein's isoelectric point (pI) based on microfluidic free-flow electrophoresis (μFFE). The approach exploits temporal rather than spatial pH gradients. To demonstrate the effectiveness of this method the pI of 7 different proteins of known pI were tested; β-lactoglobulin, ribonuclease A, ovalbumin, human transferrin, ubiquitin and myoglobin. The paper shows that this method is successful in determining the pI using this new technique without the requirement of generating and maintaining pH gradients which is often challenging for other techniques. The technique requires low voltages and low sample consumption. The paper also shows that using this technique it is possible to estimate the pI values for a wide range of proteins measuring at only two pH values, suggesting that this technique is rapid and accurate on small volume samples.
Blog04 July 2019The Protein Society Symposium 2019 roundup
In this blog post we showcase videos of authors taking us through their poster presentations and highlight key papers from the talks from our favourite seven topics.
Blog04 February 2019Protein aggregation - why it matters, and how to study it
A growing field of research is dedicated to protein aggregation, which can be useful, functional or unwanted. This article explores some of the reasons why proteins aggregate, and details the techniques available to study it.