Documentation31 July 2019Protein labelling procedure using Alexa Fluor 488 NHS ester
Long form protein labelling protocol using Alexa Fluor 488 NHS Ester from Thermo Fisher Scientific. Includes troubleshooting, purification steps and an example calculation to help with concentration and volume calculations.
Publication25 July 2019Microfluidic diffusional sizing (MDS) in the literature
Document referencing all the publications where microfluidic diffusional sizing (MDS) has been used.
Publication24 July 2019Putative interaction site for membrane phospholipids controls activation of TRPA1 channel at physiological membrane potentials.
Macikova et al use Microfluidic Diffusional Sizing (MDS) along with other techniques to understand the role phosphatidylinositol-4,5-bisphosphate (PIP2) has in the regulation of transient receptor potential ankyrin 1 (TRPA1) channel. The Fluidity One was used to create binding curves of the interactions between two specific peptides (L992-N1008 and T1003-P1034) and model lipid membranes in the presence of PIP2.
Blog02 April 2019When protein-lipid interactions matter
This blog explores some examples from the literature where the interactions of proteins and lipids have made a big impact on the overall research.
Application note02 April 2019Determination of lipid protein binding stoichiometry using Microfluidic Diffusional Sizing
Here we assess the stoichiometry of α-synuclein binding to lipid vesicles by microfluidic diffusional sizing (MDS) on the Fluidity One and Circular Dichroism (CD). We find that the results obtained are consistent between the two techniques, but that MDS presents advantages in both speed and simplicity.
Publication06 March 2019α-Synuclein-derived lipoparticles in the study of α-Synuclein amyloid fibril formation
This paper probes the interactions of α-synuclein with lipid particles using the Fluidity One and various other methods, to investigate the link between these interactions and α-synuclein aggregation.
Blog06 February 2019Born Slippy; Protein-Lipid interactions around the human body
There are many proteins which interact with lipids in vivo, in complex relationships that have crucial impacts on organism health. These are often thought of as disease causing, but can also play roles in normal biochemical functions. Here are some examples of protein-lipid interactions from around the human body, which play a part in healthy activity in cells.