Application note11 September 2019Measuring the binding affinity of protein–protein interactions in simple and complex backgrounds
Here the Fluidity One-W is used to experimentally determine the KD of protein-antibody interactions in both buffer and cell culture medium with high precision.
Application note03 April 2019Determination of KD of aptamer protein interactions by microfluidic diffusional sizing
Here the Fluidity One-W is used to experimentally determine the KD of two protein aptamer interactions, with results in good agreement with literature values. Importantly the technique required no calibration, complex setup or specialist sample preparation. Furthermore, the interaction is assessed without immobilising or altering either molecule beyond the addition of a standard fluorescent label.
Application note02 April 2019Determination of lipid protein binding stoichiometry using Microfluidic Diffusional Sizing
Here we assess the stoichiometry of α-synuclein binding to lipid vesicles by microfluidic diffusional sizing (MDS) on the Fluidity One and Circular Dichroism (CD). We find that the results obtained are consistent between the two techniques, but that MDS presents advantages in both speed and simplicity.