Resources

Publications, application notes and more

  • Blog14 August 2019

    Getting to the heart of the matter: GPCRs as therapeutic targets for heart failure

    In this blog post we cover the functions of key GPCRs in the heart and how they are now being targeted for therapeutic treatments to reduce the impact of coronary heart disease

  • Video13 August 2019

    Conference Poster Presentations 2019

    Throughout 2019 we will be attending conferences all over the world and seeking out research topics and poster presetations we really think are worth taking a look at. Check back here regularly for videos of poster presentations that present cutting edge science from around the globe.

  • Documentation31 July 2019

    Protein labelling procedure using Alexa Fluor 488 NHS ester

    Long form protein labelling protocol using Alexa Fluor 488 NHS Ester from Thermo Fisher Scientific. Includes troubleshooting, purification steps and an example calculation to help with concentration and volume calculations.

  • Documentation30 July 2019

    Quick Guide: Labelling with Alexa Fluor 488 NHS Ester

    Quick guide to label and purify proteins with Alexa Fluor 488 NHS ester for use with the Fluidity One-W.

  • Publication25 July 2019

    Gradient-free determination of isoelectric points of proteins on chip

    Lapinska et al. using a microfluidic system built in house design a new technique to determine a protein's isoelectric point (pI) based on microfluidic free-flow electrophoresis (μFFE). The approach exploits temporal rather than spatial pH gradients. To demonstrate the effectiveness of this method the pI of 7 different proteins of known pI were tested; β-lactoglobulin, ribonuclease A, ovalbumin, human transferrin, ubiquitin and myoglobin. The paper shows that this method is successful in determining the pI using this new technique without the requirement of generating and maintaining pH gradients which is often challenging for other techniques. The technique requires low voltages and low sample consumption. The paper also shows that using this technique it is possible to estimate the pI values for a wide range of proteins measuring at only two pH values, suggesting that this technique is rapid and accurate on small volume samples.

  • Publication25 July 2019

    Microfluidic diffusional sizing (MDS) in the literature

    Document referencing all the publications where microfluidic diffusional sizing (MDS) has been used.

  • Publication24 July 2019

    Putative interaction site for membrane phospholipids controls activation of TRPA1 channel at physiological membrane potentials.

    Macikova et al use Microfluidic Diffusional Sizing (MDS) along with other techniques to understand the role phosphatidylinositol-4,5-bisphosphate (PIP2) has in the regulation of transient receptor potential ankyrin 1 (TRPA1) channel. The Fluidity One was used to create binding curves of the interactions between two specific peptides (L992-N1008 and T1003-P1034) and model lipid membranes in the presence of PIP2.

  • Blog04 July 2019

    The Protein Society Symposium 2019 roundup

    In this blog post we showcase videos of authors taking us through their poster presentations and highlight key papers from the talks from our favourite seven topics.

  • Blog03 July 2019

    Affinity vs Avidity- What's the difference?

    In this blog post we will be delving into the differences between affinity and avidity

  • Publication04 June 2019

    Secondary nucleation and elongation occur at different sites on Alzheimer’s amyloid-β aggregates

    In this paper MDS was used to identify two molecular chaperones which suppress secondary nucleation processes and inhibit the elongation of Aβ fibrils seen in Alzheimer's disease.

  • Blog24 May 2019

    5 great reads: Protein-protein interactions and disease

    Here are five of our favorite recent publications, highlighting both the role of protein-protein interactions in disease and how the characterization of these interactions may allow the development of new therapeutic strategies.

  • Application note02 April 2019

    Determination of lipid protein binding stoichiometry using Microfluidic Diffusional Sizing

    Here we assess the stoichiometry of α-synuclein binding to lipid vesicles by microfluidic diffusional sizing (MDS) on the Fluidity One and Circular Dichroism (CD). We find that the results obtained are consistent between the two techniques, but that MDS presents advantages in both speed and simplicity.

  • Application note01 April 2019

    Determination of KD of aptamer protein interactions by microfluidic diffusional sizing

    Here the Fluidity One-W is used to experimentally determine the KD of two protein aptamer interactions, with results in good agreement with literature values. Importantly the technique required no calibration, complex setup or specialist sample preparation. Furthermore, the interaction is assessed without immobilising or altering either molecule beyond the addition of a standard fluorescent label.

  • Infographic07 March 2019

    The KD cheat sheet

    This printable "cheat sheet" gives an overview of what KD, the dissociation constant, means, how to calculate it, and how to experimentally measure it.

  • Publication06 March 2019

    α-Synuclein-derived lipoparticles in the study of α-Synuclein amyloid fibril formation

    This paper probes the interactions of α-synuclein with lipid particles using the Fluidity One and various other methods, to investigate the link between these interactions and α-synuclein aggregation.

  • Blog06 February 2019

    Born Slippy; Protein-Lipid interactions around the human body

    There are many proteins which interact with lipids in vivo, in complex relationships that have crucial impacts on organism health. These are often thought of as disease causing, but can also play roles in normal biochemical functions. Here are some examples of protein-lipid interactions from around the human body, which play a part in healthy activity in cells.