Application noteDetermination of lipid protein binding stoichiometry using Microfluidic Diffusional Sizing
Here we assess the stoichiometry of α-synuclein binding to lipid vesicles by microfluidic diffusional sizing (MDS) on the Fluidity One and Circular Dichroism (CD). We find that the results obtained are consistent between the two techniques, but that MDS presents advantages in both speed and simplicity.
Application noteDetermination of KD of aptamer protein interactions by microfluidic diffusional sizing
Here the Fluidity One-W is used to experimentally determine the KD of two protein aptamer interactions, with results in good agreement with literature values. Importantly the technique required no calibration, complex setup or specialist sample preparation. Furthermore, the interaction is assessed without immobilising or altering either molecule beyond the addition of a standard fluorescent label.
InfographicThe KD cheat sheet
This printable "cheat sheet" gives an overview of what KD, the dissociation constant, means, how to calculate it, and how to experimentally measure it.
Publicationα-Synuclein-derived lipoparticles in the study of α-Synuclein amyloid fibril formation
This paper probes the interactions of α-synuclein with lipid particles using the Fluidity One and various other methods, to investigate the link between these interactions and α-synuclein aggregation.
BlogBorn Slippy; Protein-Lipid interactions around the human body
There are many proteins which interact with lipids in vivo, in complex relationships that have crucial impacts on organism health. These are often thought of as disease causing, but can also play roles in normal biochemical functions. Here are some examples of protein-lipid interactions from around the human body, which play a part in healthy activity in cells.