Blog23 August 2019Looking at the numbers: Why protein stoichiometry matters
Accurate determination of stoichiometry for a protein complex is critical to our understanding how the complex will react to other proteins or small molecules. Here we look at how stoichiometry not only effects important viral protein-antibody interactions but also disease pathology.
Video13 August 2019Conference Poster Presentations 2019
Throughout 2019 we will be attending conferences all over the world and seeking out research topics and poster presetations we really think are worth taking a look at. Check back here regularly for videos of poster presentations that present cutting edge science from around the globe.
Video12 August 2019Measuring IL2 Stability in different buffers using Microfluidic Diffusional Sizing (MDS)
In this video we look at how a commercially available human interleukin-2 is assessed by microfluidic diffusional sizing on the Fluidity One across a dilution series. The hydrodynamic radius is observed to increase with increasing concentration, in a way which suggests a monomer-trimer equilibrium with positive cooperativity is established.
Webinar22 March 2019Using MDS to observe protein oligomerization
In this webinar we explore protein oligomerization, and look at two case studies where microfluidic diffusional sizing (MDS) was used to observe changing oligomeric states.
Application note31 October 2018Protein Size as an Indication of Structure
Molecular weight (Mw) is a commonly used, and for many scientists a readily understood, parameter to describe the size of a protein or complex. Here we show how hydrodynamic radius (Rh) can be used in combination with Mw to provide insights into the shape and structure of proteins and illustrate how Mw alone may not always provide a complete picture.
Application note22 August 2018Oligomerization of Interleukin-2
A commercially available human interleukin-2 is assessed by microfluidic diffusional sizing on the Fluidity One across a dilution series. The hydrodynamic radius is observed to increase with increasing concentration, in a way which suggests a monomer-trimer equilibrium with positive cooperativity is established.
Publication15 June 2018Cooperative Assembly of Hsp70 Subdomain Clusters
Wright et al use Microfluidic Diffusional Sizing to probe the oligomerisation of the SBD641 substrate of human Hsp70. The Fluidity One was employed to verify if the fluorescent label used had an affect on measured hydrodynamic radius during the tests.
Application note10 May 2018Detecting insulin oligomerization using microfluidic diffusional sizing
Insulin monomers self-assemble into hexamers, which is known to affect its level of uptake in the human body. Here we show that Microfluidic Diffusional Sizing (MDS) can be used to detect these changes.