Resources

Publications, application notes and more

  • Application note

    Protein Size as an Indication of Structure

    Molecular weight (Mw) is a commonly used, and for many scientists a readily understood, parameter to describe the size of a protein or complex. Here we show how hydrodynamic radius (Rh) can be used in combination with Mw to provide insights into the shape and structure of proteins and illustrate how Mw alone may not always provide a complete picture.

  • Documentation

    Fluidity One Chip Flow factsheet

    This factsheet shows how the microfluidic chips used with the Fluidity One work, through diagrams and a short description.

  • Video

    Introducing The Fluidity One - video

    This short video introduces the Fluidity One instrument, how it works, what Microfluidic Diffusional Sizing MDS is, and why native state protein analysis is so vital.

  • Blog

    Basel Life and Miptec 2018 - poster and presentation download

    The Miptec exhibition at Basel Life 2018 marked the official launch of our Fluidity One instrument. Download a copy of our poster and presentation from the event here.

  • Brochure

    Fluidity One brochure

    The Fluidity One brochure gives an overview of the capabilities, applications and full technical specifications.

  • Application note

    Oligomerization of Interleukin-2

    A commercially available human interleukin-2 is assessed by microfluidic diffusional sizing on the Fluidity One across a dilution series. The hydrodynamic radius is observed to increase with increasing concentration, in a way which suggests a monomer-trimer equilibrium with positive cooperativity is established.

  • Application note

    Interleukin-2 stability in changing buffer and temperature conditions

    The stability of interleukin-2 in different buffers and storage temperatures is evaluated using the Fluidity One. We find that IL-2 forms aggregates within 24 hours in some buffers, and that the Fluidity One provides a simple means to evaluate the stability of proteins across different conditions.

  • Publication

    Cooperative Assembly of Hsp70 Subdomain Clusters

    Wright et al use Microfluidic Diffusional Sizing to probe the oligomerisation of the SBD641 substrate of human Hsp70. The Fluidity One was employed to verify if the fluorescent label used had an affect on measured hydrodynamic radius during the tests.

  • Application note

    A comparison of Microfluidic Diffusional Sizing with Dynamic Light Scattering and Taylor Dispersion Analysis

    The established technologies of Dynamic Light Scattering (DLS) and Taylor Dispersion Analysis (TDA) are compared to Microfluidic Diffusional Sizing (MDS) for sizing proteins of varying molecular weights and at varying concentrations. We show that MDS offers comparable sizing of proteins over a range of sizes, and can provide consistent sizing to lower concentrations than the other techniques.

  • Blog

    How does the Fluidity One compare to UV-Vis spectrophotometers in testing the concentration of proteins?

    UV-vis absorbance testing is a popular method for rapid testing of protein concentration with only a small sample volume needed. Here we compare this method to Microfluidic Diffusional Sizing (MDS), as used on the Fluidity One, to see how the workflow, accuracy, test method, range and limitations compare.

  • Blog

    Protein size - how do I measure it, and why is it important?

    An overview of why protein size matters, and what structural and functional information protein size can reveal. To understand proteins and their function, we have to understand the way they fold, aggregate and interact. Conformation is key to protein function and can be revealed by measuring size. Different methods for measuring protein size are summarised, and comparison is made, considering the method, range, cost and limitations of each technology.

  • Application note

    Detecting insulin oligomerization using microfluidic diffusional sizing

    Insulin monomers self-assemble into hexamers, which is known to affect its level of uptake in the human body. Here we show that Microfluidic Diffusional Sizing (MDS) can be used to detect these changes.

  • Blog

    What analytical laboratory methods are available for protein quantification? How do they compare?

    Quantification is an essential part of protein analysis and quality control. Here we look at the various approaches for protein quantification, their advantages, and their limitations.