Resources

Publications, application notes and more

  • Publication25 July 2019

    Microfluidic diffusional sizing (MDS) in the literature

    Document referencing all the publications where microfluidic diffusional sizing (MDS) has been used.

  • Publication24 July 2019

    Putative interaction site for membrane phospholipids controls activation of TRPA1 channel at physiological membrane potentials.

    Macikova et al use Microfluidic Diffusional Sizing (MDS) along with other techniques to understand the role phosphatidylinositol-4,5-bisphosphate (PIP2) has in the regulation of transient receptor potential ankyrin 1 (TRPA1) channel. The Fluidity One was used to create binding curves of the interactions between two specific peptides (L992-N1008 and T1003-P1034) and model lipid membranes in the presence of PIP2.

  • Publication04 June 2019

    Secondary nucleation and elongation occur at different sites on Alzheimer’s amyloid-β aggregates

    In this paper MDS was used to identify two molecular chaperones which suppress secondary nucleation processes and inhibit the elongation of Aβ fibrils seen in Alzheimer's disease.

  • Application note04 April 2019

    Rapid quality control of commercially available antibodies by MDS for acceptance testing

    Size presents a simple metric to assess quality as an indicator of aggregation and degradation. Here MDS on the Fluidity One and SEC analysis are both used to check 6 commercially available antibodies. Both techniques sucessfully identify problem samples, but MDS does so with 80% less time and 90% less sample.

  • Webinar22 March 2019

    Using MDS to observe protein oligomerization

    In this webinar we explore protein oligomerization, and look at two case studies where microfluidic diffusional sizing (MDS) was used to observe changing oligomeric states.

  • Publication06 March 2019

    α-Synuclein-derived lipoparticles in the study of α-Synuclein amyloid fibril formation

    This paper probes the interactions of α-synuclein with lipid particles using the Fluidity One and various other methods, to investigate the link between these interactions and α-synuclein aggregation.

  • Documentation11 February 2019

    Fluidity One quick start guide

    This handy quick start guide will have you running tests and interpreting results on the Fluidity One as soon as possible.

  • Documentation02 January 2019

    Fluidity One User Manual

    The Fluidity One user manual with full safety and operation information.

  • Application note11 December 2018

    Quantitation of low Tryptophan and Tyrosine peptides using the Fluidity One

    UV absorbance is commonly used as a fast method for protein and peptide quantitation, however samples must contain tryptophan (Trp) or tyrosine (Tyr) residues to be detected. Here we demonstrate the ability of the Fluidity One to assess the concentration of a range of samples which have low or no Trp and Tyr content.

  • Application note31 October 2018

    Protein Size as an Indication of Structure

    Molecular weight (Mw) is a commonly used, and for many scientists a readily understood, parameter to describe the size of a protein or complex. Here we show how hydrodynamic radius (Rh) can be used in combination with Mw to provide insights into the shape and structure of proteins and illustrate how Mw alone may not always provide a complete picture.

  • Documentation26 September 2018

    Fluidity One Chip Flow factsheet

    This factsheet shows how the microfluidic chips used with the Fluidity One work, through diagrams and a short description.

  • Video18 September 2018

    Introducing The Fluidity One - video

    This short video introduces the Fluidity One instrument, how it works, what Microfluidic Diffusional Sizing MDS is, and why native state protein analysis is so vital.

  • Blog14 September 2018

    Basel Life and Miptec 2018 - poster and presentation download

    The Miptec exhibition at Basel Life 2018 marked the official launch of our Fluidity One instrument. Download a copy of our poster and presentation from the event here.

  • Brochure24 August 2018

    Fluidity One brochure

    The Fluidity One brochure gives an overview of the capabilities, applications and full technical specifications.

  • Application note22 August 2018

    Oligomerization of Interleukin-2

    A commercially available human interleukin-2 is assessed by microfluidic diffusional sizing on the Fluidity One across a dilution series. The hydrodynamic radius is observed to increase with increasing concentration, in a way which suggests a monomer-trimer equilibrium with positive cooperativity is established.

  • Application note22 August 2018

    Interleukin-2 stability in changing buffer and temperature conditions

    The stability of interleukin-2 in different buffers and storage temperatures is evaluated using the Fluidity One. We find that IL-2 forms aggregates within 24 hours in some buffers, and that the Fluidity One provides a simple means to evaluate the stability of proteins across different conditions.

  • Publication15 June 2018

    Cooperative Assembly of Hsp70 Subdomain Clusters

    Wright et al use Microfluidic Diffusional Sizing to probe the oligomerisation of the SBD641 substrate of human Hsp70. The Fluidity One was employed to verify if the fluorescent label used had an affect on measured hydrodynamic radius during the tests.

  • Application note14 June 2018

    A comparison of Microfluidic Diffusional Sizing with Dynamic Light Scattering and Taylor Dispersion Analysis

    The established technologies of Dynamic Light Scattering (DLS) and Taylor Dispersion Analysis (TDA) are compared to Microfluidic Diffusional Sizing (MDS) for sizing proteins of varying molecular weights and at varying concentrations. We show that MDS offers comparable sizing of proteins over a range of sizes, and can provide consistent sizing to lower concentrations than the other techniques.

  • Blog30 May 2018

    How does the Fluidity One compare to UV-Vis spectrophotometers in testing the concentration of proteins?

    UV-vis absorbance testing is a popular method for rapid testing of protein concentration with only a small sample volume needed. Here we compare this method to Microfluidic Diffusional Sizing (MDS), as used on the Fluidity One, to see how the workflow, accuracy, test method, range and limitations compare.

  • Blog17 May 2018

    Protein size - how do I measure it, and why is it important?

    An overview of why protein size matters, and what structural and functional information protein size can reveal. To understand proteins and their function, we have to understand the way they fold, aggregate and interact. Conformation is key to protein function and can be revealed by measuring size.

    Different methods for measuring protein size are summarised, and comparison is made, considering the method, range, cost and limitations of each technology.

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