Resources

Publications, application notes and more

  • Blog23 August 2019

    Looking at the numbers: Why protein stoichiometry matters

    Accurate determination of stoichiometry for a protein complex is critical to our understanding how the complex will react to other proteins or small molecules. Here we look at how stoichiometry not only effects important viral protein-antibody interactions but also disease pathology.

  • Video20 August 2019

    Application note video summaries

    On this page we collect short video summaries of our application notes

  • Blog14 August 2019

    Getting to the heart of the matter: GPCRs as therapeutic targets for heart failure

    In this blog post we cover the functions of key GPCRs in the heart and how they are now being targeted for therapeutic treatments to reduce the impact of coronary heart disease

  • Video13 August 2019

    Conference Poster Presentations 2019

    Throughout 2019 we will be attending conferences all over the world and seeking out research topics and poster presetations we really think are worth taking a look at. Check back here regularly for videos of poster presentations that present cutting edge science from around the globe.

  • Video12 August 2019

    Measuring IL2 Stability in different buffers using Microfluidic Diffusional Sizing (MDS)

    In this video we look at how a commercially available human interleukin-2 is assessed by microfluidic diffusional sizing on the Fluidity One across a dilution series. The hydrodynamic radius is observed to increase with increasing concentration, in a way which suggests a monomer-trimer equilibrium with positive cooperativity is established.

  • Publication01 August 2019

    Analysis of αB-crystallin polydispersity in solution through native microfluidic electrophoresis

    In this paper, the authors utilise microfluidic diffusional sizing and electrophoresis to characterise the oligomeric states of the chaperone protein alphaB-crystallin.

  • Documentation31 July 2019

    Protein labelling procedure using Alexa Fluor 488 NHS ester

    Long form protein labelling protocol using Alexa Fluor 488 NHS Ester from Thermo Fisher Scientific. Includes troubleshooting, purification steps and an example calculation to help with concentration and volume calculations.

  • Documentation30 July 2019

    Quick Guide: Labelling with Alexa Fluor 488 NHS Ester

    Quick guide to label and purify proteins with Alexa Fluor 488 NHS ester for use with the Fluidity One-W.

  • Publication29 July 2019

    On-chip label-free protein analysis with downstream electrodes for direct removal of electrolysis products

    Saar et al designed and demonstrated a single step lithography approach to fabricate microfluidic devices that can be used to apply high electric fields without the formation of any electrolysis products in the electrophoresis area.

  • Video25 July 2019

    KD video series

    Here is our series of videos that explain what te dissociation constant, KD, is and how to derrive it on a binding curve.

  • Publication25 July 2019

    Gradient-free determination of isoelectric points of proteins on chip

    Lapinska et al. using a microfluidic system built in house design a new technique to determine a protein's isoelectric point (pI) based on microfluidic free-flow electrophoresis (μFFE). The approach exploits temporal rather than spatial pH gradients. To demonstrate the effectiveness of this method the pI of 7 different proteins of known pI were tested; β-lactoglobulin, ribonuclease A, ovalbumin, human transferrin, ubiquitin and myoglobin. The paper shows that this method is successful in determining the pI using this new technique without the requirement of generating and maintaining pH gradients which is often challenging for other techniques. The technique requires low voltages and low sample consumption. The paper also shows that using this technique it is possible to estimate the pI values for a wide range of proteins measuring at only two pH values, suggesting that this technique is rapid and accurate on small volume samples.

  • Publication25 July 2019

    Microfluidic diffusional sizing (MDS) in the literature

    Document referencing all the publications where microfluidic diffusional sizing (MDS) has been used.

  • Video24 July 2019

    Lucie Machikova - EBSA IUPAP 2019 Poster video

    Lucie Machikova talking through her poster detailing the work carried out in her publication "Putative interaction site for membrane phospholipids controls activation of TRPA1 channel at physiological membrane potentials".

  • Publication24 July 2019

    Putative interaction site for membrane phospholipids controls activation of TRPA1 channel at physiological membrane potentials.

    Macikova et al use Microfluidic Diffusional Sizing (MDS) along with other techniques to understand the role phosphatidylinositol-4,5-bisphosphate (PIP2) has in the regulation of transient receptor potential ankyrin 1 (TRPA1) channel. The Fluidity One was used to create binding curves of the interactions between two specific peptides (L992-N1008 and T1003-P1034) and model lipid membranes in the presence of PIP2.

  • Blog11 July 2019

    Understanding why GPCRs are difficult to study

    In this blog post we delve into the reasons behind why elucidating GPCR structures are so important as well as why they are hard to study.

  • Infographic11 July 2019

    Researching therapies for Alzheimer’s Disease

    In this infographic, we break down all the failures of potential therapies that were tested to treat Alzheimer's Disease. This includes a cost and percentage breakdown of the clinical phases in which these therapies failed in as well as statistics for world-wide Alzherimer's Disease epidemiology.

  • Blog04 July 2019

    The Protein Society Symposium 2019 roundup

    In this blog post we showcase videos of authors taking us through their poster presentations and highlight key papers from the talks from our favourite seven topics.

  • Blog03 July 2019

    Affinity vs Avidity- What's the difference?

    In this blog post we will be delving into the differences between affinity and avidity

  • Brochure27 June 2019

    Fluidity One-W Brochure

    This brochure gives an overview of the capabilities, applications and full technical specifications of the Fluidity One-W

  • Publication04 June 2019

    Secondary nucleation and elongation occur at different sites on Alzheimer’s amyloid-β aggregates

    In this paper MDS was used to identify two molecular chaperones which suppress secondary nucleation processes and inhibit the elongation of Aβ fibrils seen in Alzheimer's disease.

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