Application note

A comparison of Microfluidic Diffusional Sizing with Dynamic Light Scattering and Taylor Dispersion Analysis

Here we compare a number of well-established methods exist for measuring the size of proteins, such as Dynamic Light Scattering (DLS) and Taylor Dispersion Analysis (TDA) against microfluidic diffusional sizing (MDS).

  • When measuring hydrodynamic radius MDS analysis is shown to provide accurate data across a range of protein sizes which correlates with results obtained using other techniques.
  • In testing samples across a concentration range, results obtained by MDS were consistent even at low concentrations (<50 μg/mL), while measurements at these concentrations were either not possible with TDA or were inaccurate with DLS.
  • In summary, protein analysis by MDS on the Fluidity One offers an orthogonal data point and is a robust alternative to DLS and TDA for determination of unlabelled protein size in solution.

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