Application note

Interleukin-2 stability in changing buffer and temperature conditions

The ability to conduct reliable and repeatable experiments on protein molecules relies on beginning with consistent and stable samples. A wide range of factors can affect protein stability, including temperature, pH, salt concentration, preservatives and cosolutes. Finding the optimum storage conditions for a protein of interest is the essential first step to conducting robust studies and analyses.



  • The Fluidity One provides a simple means to evaluate stability over varying buffer and temperature conditions, which could be employed when working with new proteins and peptides, or could be employed immediately prior to use of critical samples to ensure their integrity.
  • From the results it appears that IL-2 forms aggregates within 24 hrs when prepared in PBS, but not when prepared
    in acetic acid. Temperature was found to have little effect on the stability of samples incubated in acetic acid.


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