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Fluidity One-W User manual — software version 3.6.0

The User manual for the Fluidity One-W. This is for software version 3.6.0.

The Fluidity One-W uses Microfluidic Diffusional Sizing (MDS) to size labelled species, such
as proteins, in solution in less than 15 minutes. Titrating a binding partner against the
labelled species can be used to determine binding affinity, giving a KD value as well as the
size (Rh) of the labelled species and the complex. This can be used in a laboratory
environment for analysis of protein interactions, stochiometry, protein conformation, and
aggregation. It can even perform analysis on proteins in complex biological backgrounds,
such as cell lysates.