BlogWhat analytical laboratory methods are available for protein quantification? How do they compare?
Quantification is an essential part of protein analysis and quality control. Here we look at the various approaches for protein quantification, their advantages, and their limitations.
PublicationA microfluidic diffusion platform for characterizing the size of lipid vesicles and the thermodynamics of protein-lipid interactions
Hongze Gang in the Tuomas Knowles lab demonstrates the use of microfluidic diffusional sizing for characterizing the size of lipid vesicles and the thermodynamics of protein-lipid interactions.
PublicationA Microfluidic Platform for Real-Time Detection and Quantification of Protein-Ligand Interactions
In this paper, Herling et al demonstrate a microfluidic platform for the detection of protein-ligand interactions with an assay time on the second timescale and without the requirement for immobilization or the presence of a highly viscous matrix.
PublicationProtein Aggregate-Ligand Binding Assays Based on Microfluidic Diffusional Separation
Zhang et al again demonstrate the utility of Microfluidic Diffusional Sizing to monitor Nanobody binding to ɑ-synuclein, but this time also use MDS to monitor ɑ-synuclein fibril formation.
PublicationMicrofluidic diffusion viscometer for rapid analysis of complex solutions.
Arosio et al demonstrate the utility of a diffusion viscometer, showing that by including tracer particles and tracking mass transport, the viscosity of complex solutions can be determined using only low quantities of sample material, with a short analysis time and can be integrated into other microfluidic elements on a chip.
PublicationLatent analysis of unmodified biomolecules and their complexes in solution with attomole detection sensitivity
In this paper, Yates et al show that the technology behind Fluidic Analytics instruments - microfludic diffusional sizing - can be used to accurately size a range of proteins.
PublicationMicrofluidic diffusion analysis of the sizes and interactions of proteins under native solution conditions.
In this paper from Arosio et al microfluidic diffusional sizing (MDS) is used in combination with pre-labelled fluorescent biomolecules to size and quantify proteins in complex mixtures. Initially, MDS is compared to Dynamic Light Scatter (DLS) - and a good correlation is observed for monodispersed solutions.