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Blog
What biophysical attributes of biopharmaceuticals are measured, and why?An array of characteristics must be investigated in new biopharmaceuticals to evaluate their safety, efficacy and behaviour under changing conditions. Here we look at what specific attributes should be measured and why.
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Blog
A day in the life of a Software Engineer at Fluidic AnalyticsPrincipal Software Engineer, Mauricio Varea, talks about his career path to date, working in large and small companies and what a typical day looks like for him at Fluidic Analytics.
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Blog
How does Microfluidic Diffusional Sizing (MDS) compare to Dynamic Light Scattering (DLS) for protein size tests?Measuring the size of proteins provides insights into folding and conformations, aggregation and oligomerization. We compare and contrast the benefits and limitations of two popular techniques - DLS Dynamic Light Scattering, and MDS Microfluidic Diffusional Sizing.
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Blog
A day in the life of a scientist at Fluidic AnalyticsAn interview with Dr Maren Butz, senior scientist at Fluidic Analytics. We find out what an average day looks like for her, how work in academia compares to industry, and how her work contributes to developing our instruments.
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Publication
Cooperative Assembly of Hsp70 Subdomain ClustersWright et al use Microfluidic Diffusional Sizing to probe the oligomerisation of the SBD641 substrate of human Hsp70. The Fluidity One was employed to verify if the fluorescent label used had an affect on measured hydrodynamic radius during the tests.
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Application note
A comparison of Microfluidic Diffusional Sizing with Dynamic Light Scattering and Taylor Dispersion AnalysisThe established technologies of Dynamic Light Scattering (DLS) and Taylor Dispersion Analysis (TDA) are compared to Microfluidic Diffusional Sizing (MDS) for sizing proteins of varying molecular weights and at varying concentrations. We show that MDS offers comparable sizing of proteins over a range of sizes, and can provide consistent sizing to lower concentrations than the other techniques.
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Blog
How does the Fluidity One compare to UV-Vis spectrophotometers in testing the concentration of proteins?UV-vis absorbance testing is a popular method for rapid testing of protein concentration with only a small sample volume needed. Here we compare this method to Microfluidic Diffusional Sizing (MDS), as used on the Fluidity One, to see how the workflow, accuracy, test method, range and limitations compare.
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Blog
Protein size - how do I measure it, and why is it important?An overview of why protein size matters, and what structural and functional information protein size can reveal. To understand proteins and their function, we have to understand the way they fold, aggregate and interact. Conformation is key to protein function and can be revealed by measuring size. Different methods for measuring protein size are summarised, and comparison is made, considering the method, range, cost and limitations of each technology.
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Application note
Detecting insulin oligomerisation using microfluidic diffusional sizingInsulin monomers self-assemble into hexamers, which is known to affect its level of uptake in the human body. Here we show that Microfluidic Diffusional Sizing (MDS) can be used to detect these changes.
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Blog
What analytical laboratory methods are available for protein quantification? How do they compare?Quantification is an essential part of protein analysis and quality control. Here we look at the various approaches for protein quantification, their advantages, and their limitations.
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Publication
A microfluidic diffusion platform for characterizing the size of lipid vesicles and the thermodynamics of protein-lipid interactionsHongze Gang in the Tuomas Knowles lab demonstrates the use of microfluidic diffusional sizing for characterizing the size of lipid vesicles and the thermodynamics of protein-lipid interactions.
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Application note
Comparison of microfluidic diffusional sizing and ThT spectroscopy for the assessment of α-synuclein amyloid fibril growthThe aggregation of α-Synuclein results in insoluble amyloid fibrils. Here we show that Microfluidic Diffusional Sizing (MDS) offers a new approach to study these aggregations in their native state.
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Publication
A Microfluidic Platform for Real-Time Detection and Quantification of Protein-Ligand InteractionsIn this paper, Herling et al demonstrate a microfluidic platform for the detection of protein-ligand interactions with an assay time on the second timescale and without the requirement for immobilization or the presence of a highly viscous matrix.
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Publication
Protein Aggregate-Ligand Binding Assays Based on Microfluidic Diffusional SeparationZhang et al again demonstrate the utility of Microfluidic Diffusional Sizing to monitor Nanobody binding to ɑ-synuclein, but this time also use MDS to monitor ɑ-synuclein fibril formation.
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Publication
Microfluidic diffusion viscometer for rapid analysis of complex solutions.Arosio et al demonstrate the utility of a diffusion viscometer, showing that by including tracer particles and tracking mass transport, the viscosity of complex solutions can be determined using only low quantities of sample material, with a short analysis time and can be integrated into other microfluidic elements on a chip.
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Publication
Latent analysis of unmodified biomolecules and their complexes in solution with attomole detection sensitivityIn this paper, Yates et al show that the technology behind Fluidic Analytics instruments - microfludic diffusional sizing - can be used to accurately size a range of proteins.
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Publication
Microfluidic diffusion analysis of the sizes and interactions of proteins under native solution conditions.In this paper from Arosio et al microfluidic diffusional sizing (MDS) is used in combination with pre-labelled fluorescent biomolecules to size and quantify proteins in complex mixtures. Initially, MDS is compared to Dynamic Light Scatter (DLS) - and a good correlation is observed for monodispersed solutions.