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Documentation
Fluidity One Chip Flow factsheetThis factsheet shows how the microfluidic chips used with the Fluidity One work, through diagrams and a short description.
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Video
Introducing The Fluidity One - videoThis short video introduces the Fluidity One instrument, how it works, what Microfluidic Diffusional Sizing MDS is, and why native state protein analysis is so vital.
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Blog
Basel Life and Miptec 2018 - poster and presentation downloadThe Miptec exhibition at Basel Life 2018 marked the official launch of our Fluidity One instrument. Download a copy of our poster and presentation from the event here.
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Brochure
Fluidity One brochureThe Fluidity One brochure gives an overview of the capabilities, applications and full technical specifications.
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Application note
Oligomerization of Interleukin-2A commercially available human interleukin-2 is assessed by microfluidic diffusional sizing on the Fluidity One across a dilution series. The hydrodynamic radius is observed to increase with increasing concentration, in a way which suggests a monomer-trimer equilibrium with positive cooperativity is established.
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Application note
Interleukin-2 stability in changing buffer and temperature conditionsThe stability of interleukin-2 in different buffers and storage temperatures is evaluated using the Fluidity One. We find that IL-2 forms aggregates within 24 hours in some buffers, and that the Fluidity One provides a simple means to evaluate the stability of proteins across different conditions.
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Blog
What biophysical attributes of biopharmaceuticals are measured, and why?An array of characteristics must be investigated in new biopharmaceuticals to evaluate their safety, efficacy and behaviour under changing conditions. Here we look at what specific attributes should be measured and why.
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Blog
A day in the life of a Software Engineer at Fluidic AnalyticsPrincipal Software Engineer, Mauricio Varea, talks about his career path to date, working in large and small companies and what a typical day looks like for him at Fluidic Analytics.
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Blog
How does Microfluidic Diffusional Sizing (MDS) compare to Dynamic Light Scattering (DLS) for protein size tests?Measuring the size of proteins provides insights into folding and conformations, aggregation and oligomerization. We compare and contrast the benefits and limitations of two popular techniques - DLS Dynamic Light Scattering, and MDS Microfluidic Diffusional Sizing.
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Blog
A day in the life of a scientist at Fluidic AnalyticsAn interview with Dr Maren Butz, senior scientist at Fluidic Analytics. We find out what an average day looks like for her, how work in academia compares to industry, and how her work contributes to developing our instruments.
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Publication
Cooperative Assembly of Hsp70 Subdomain ClustersWright et al use Microfluidic Diffusional Sizing to probe the oligomerisation of the SBD641 substrate of human Hsp70. The Fluidity One was employed to verify if the fluorescent label used had an affect on measured hydrodynamic radius during the tests.
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Application note
A comparison of Microfluidic Diffusional Sizing with Dynamic Light Scattering and Taylor Dispersion AnalysisThe established technologies of Dynamic Light Scattering (DLS) and Taylor Dispersion Analysis (TDA) are compared to Microfluidic Diffusional Sizing (MDS) for sizing proteins of varying molecular weights and at varying concentrations. We show that MDS offers comparable sizing of proteins over a range of sizes, and can provide consistent sizing to lower concentrations than the other techniques.
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Blog
How does the Fluidity One compare to UV-Vis spectrophotometers in testing the concentration of proteins?UV-vis absorbance testing is a popular method for rapid testing of protein concentration with only a small sample volume needed. Here we compare this method to Microfluidic Diffusional Sizing (MDS), as used on the Fluidity One, to see how the workflow, accuracy, test method, range and limitations compare.
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Blog
Protein size - how do I measure it, and why is it important?An overview of why protein size matters, and what structural and functional information protein size can reveal. To understand proteins and their function, we have to understand the way they fold, aggregate and interact. Conformation is key to protein function and can be revealed by measuring size. Different methods for measuring protein size are summarised, and comparison is made, considering the method, range, cost and limitations of each technology.
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Application note
Detecting insulin oligomerisation using microfluidic diffusional sizingInsulin monomers self-assemble into hexamers, which is known to affect its level of uptake in the human body. Here we show that Microfluidic Diffusional Sizing (MDS) can be used to detect these changes.
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Blog
What analytical laboratory methods are available for protein quantification? How do they compare?Quantification is an essential part of protein analysis and quality control. Here we look at the various approaches for protein quantification, their advantages, and their limitations.
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Publication
A microfluidic diffusion platform for characterizing the size of lipid vesicles and the thermodynamics of protein-lipid interactionsHongze Gang in the Tuomas Knowles lab demonstrates the use of microfluidic diffusional sizing for characterizing the size of lipid vesicles and the thermodynamics of protein-lipid interactions.
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Publication
A Microfluidic Platform for Real-Time Detection and Quantification of Protein-Ligand InteractionsIn this paper, Herling et al demonstrate a microfluidic platform for the detection of protein-ligand interactions with an assay time on the second timescale and without the requirement for immobilization or the presence of a highly viscous matrix.
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Publication
Protein Aggregate-Ligand Binding Assays Based on Microfluidic Diffusional SeparationZhang et al again demonstrate the utility of Microfluidic Diffusional Sizing to monitor Nanobody binding to ɑ-synuclein, but this time also use MDS to monitor ɑ-synuclein fibril formation.
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Publication
Microfluidic diffusion viscometer for rapid analysis of complex solutions.Arosio et al demonstrate the utility of a diffusion viscometer, showing that by including tracer particles and tracking mass transport, the viscosity of complex solutions can be determined using only low quantities of sample material, with a short analysis time and can be integrated into other microfluidic elements on a chip.