Publications, application notes and more

Application note

UV absorbance is commonly used as a fast method for protein and peptide quantitation, however samples must contain tryptophan (Trp) or tyrosine (Tyr) residues to be detected. Here we demonstrate the ability of the Fluidity One to assess the concentration of a range of samples which have low or no Trp and Tyr content.

Blog

As the $31m investment round in Fluidic Analytics is announced, Vishal Gulati of Draper Esprit reflects on how he first met the founders, why proteins matter and how our technology is set to change protein science.

Application note

Molecular weight (Mw) is a commonly used, and for many scientists a readily understood, parameter to describe the size of a protein or complex. Here we show how hydrodynamic radius (Rh) can be used in combination with Mw to provide insights into the shape and structure of proteins and illustrate how Mw alone may not always provide a complete picture.

Documentation

Documentation

This factsheet shows how the microfluidic chips used with the Fluidity One work, through diagrams and a short description.

Video

This short video introduces the Fluidity One instrument, how it works, what Microfluidic Diffusional Sizing MDS is, and why native state protein analysis is so vital.

Blog

The Miptec exhibition at Basel Life 2018 marked the official launch of our Fluidity One instrument. Download a copy of our poster and presentation from the event here.

Brochure

The Fluidity One brochure gives an overview of the capabilities, applications and full technical specifications.

Application note

A commercially available human interleukin-2 is assessed by microfluidic diffusional sizing on the Fluidity One across a dilution series. The hydrodynamic radius is observed to increase with increasing concentration, in a way which suggests a monomer-trimer equilibrium with positive cooperativity is established.

Application note

The stability of interleukin-2 in different buffers and storage temperatures is evaluated using the Fluidity One. We find that IL-2 forms aggregates within 24 hours in some buffers, and that the Fluidity One provides a simple means to evaluate the stability of proteins across different conditions.

Documentation

A laboratory protocol for removing additives that contain primary amines before testing on the Fluidity One.

Blog

An array of characteristics must be investigated in new biopharmaceuticals to evaluate their safety, efficacy and behaviour under changing conditions. Here we look at what specific attributes should be measured and why.

Blog

Principal Software Engineer, Mauricio Varea, talks about his career path to date, working in large and small companies and what a typical day looks like for him at Fluidic Analytics.

Blog

Measuring the size of proteins provides insights into folding and conformations, aggregation and oligomerization. We compare and contrast the benefits and limitations of two popular techniques - DLS Dynamic Light Scattering, and MDS Microfluidic Diffusional Sizing.

Blog

An interview with Dr Maren Butz, senior scientist at Fluidic Analytics. We find out what an average day looks like for her, how work in academia compares to industry, and how her work contributes to developing our instruments.

Publication

Wright et al use Microfluidic Diffusional Sizing to probe the oligomerisation of the SBD641 substrate of human Hsp70. The Fluidity One was employed to verify if the fluorescent label used had an affect on measured hydrodynamic radius during the tests.

Application note

The established technologies of Dynamic Light Scattering (DLS) and Taylor Dispersion Analysis (TDA) are compared to Microfluidic Diffusional Sizing (MDS) for sizing proteins of varying molecular weights and at varying concentrations. We show that MDS offers comparable sizing of proteins over a range of sizes, and can provide consistent sizing to lower concentrations than the other techniques.

Blog

UV-vis absorbance testing is a popular method for rapid testing of protein concentration with only a small sample volume needed. Here we compare this method to Microfluidic Diffusional Sizing (MDS), as used on the Fluidity One, to see how the workflow, accuracy, test method, range and limitations compare.

Blog

An overview of why protein size matters, and what structural and functional information protein size can reveal. To understand proteins and their function, we have to understand the way they fold, aggregate and interact. Conformation is key to protein function and can be revealed by measuring size. Different methods for measuring protein size are summarised, and comparison is made, considering the method, range, cost and limitations of each technology.

Application note

Insulin monomers self-assemble into hexamers, which is known to affect its level of uptake in the human body. Here we show that Microfluidic Diffusional Sizing (MDS) can be used to detect these changes.