Introducing the new fluidity one-w
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Simple processes — Prepare, plug, play
The minimal sample prep, easy-to-use design and wide dynamic range means you will spend less time setting up, and more time generating the data you need.
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Look at the hard stuff
Solution state measurement coupled with high sensitivity means you can study proteins that are problematic for other systems including membrane proteins, multi-protein complexes, and intrinsically disordered proteins.
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Study proteins without hassle
Work in crude biological mixtures with minimal preparation, while controlling for off-target binding. Gain valuable insights at the earliest possible stage.
Applications
Analyse protein interactions in complex backgrounds
Study protein-protein, protein-DNA and protein-lipid interactions in biological mixtures such as crude lysates of blood plasma. No surface binding means no artefacts so you can study proteins in near native state with no risk of non-specific binding.
Quantify stoichiometry of protein complexes
In the process of calculating the binding affinity of an interaction the Fluidity One-W provides the hydrodynamic radius (Rh) of both the unbound labelled species and the complex. The hydrodynamic radius can be used to infer the stoichiometry of the protein complex and the overall conformational arrangement in solution.
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How does the Fluidity One-W compare to other techniques
There is a number of other techniques that are currently used to determine protein binding affinity, all with their own strengths and weaknesses, the Fluidity One-W is no different. We believe it should be a tool in your arsenal to compliment other methods, but you need to know how it compares to the other techniques.
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Trial the Fluidity One-W yourself
How does the Fluidity One-W work?
Sounds like this could support your research?
Get in touch with our scientists to discuss if our technology could work for you.
Take a closer look
Fluidity One-W highlights
- In-solution, binding affinity measurement using microfluidic diffusional sizing (MDS)
- Low sample requirement — Just 5μl per sample
- High reproducibility and accuracy (even at low concentrations)
- Easy-to-use interface and consumable management
- Disposable single use chips and contained waste minimize risk of cross contamination between measurements and reduce cleaning and setup times
Fluidity One-W specifications
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Sizing
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Range (hydrodynamic radius)
0.7 – 20 nm
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Range (molecular weight)
0.5 kDa – 14 MDa
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Accuracy
± 10%
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Precision
CV < 10%
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Sensitivity
1 nM Alexa Fluor™ 488
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Operational Specifications
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Volume per measurement
5 µL
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Run time
Small proteins and peptides — 8 minutes
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Large proteins — 14 minutes
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Buffer compatibility
Compatible with pure buffer or crude lysates
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Runs per reagent cartridge
96
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Dimensions (D x W x H; cm)
40 x 40 x 43
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Detection
Fluorescence
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Label compatibility
GFP, FITC, Alexa Fluor™ 488 and equivalents
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Sounds like this could support your research?
Get in touch, our applications scientists will be happy to discuss your application requirements with you.
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