Introducing the new fluidity one-w

Simple processes — Prepare, plug, play

The minimal sample prep, easy-to-use design and wide dynamic range means you will spend less time setting up, and more time generating the data you need.
Look at the hard stuff

Solution state measurement coupled with high sensitivity means you can study proteins that are problematic for other systems including membrane proteins, multi-protein complexes, and intrinsically disordered proteins.
Study proteins without hassle

Work in crude biological mixtures with minimal preparation, while controlling for off-target binding. Gain valuable insights at the earliest possible stage.

Applications

Analyse protein interactions

Quantifying stoichiometry

Technique comparison

Analyse protein interactions

Analyse protein interactions in complex backgrounds

Study protein-protein, protein-DNA and protein-lipid interactions in biological mixtures such as crude lysates of blood plasma. No surface binding means no artefacts so you can study proteins in near native state with no risk of non-specific binding.

View application notes

Measuring the binding affinity of protein-protein interaction in simple and complex backgrounds Determination of KD of aptamer protein interactions by microfluidic diffusional sizing

Quantifying stoichiometry

Quantify stoichiometry of protein complexes

In the process of calculating the binding affinity of an interaction the Fluidity One-W provides the hydrodynamic radius (R_h) of both the unbound labelled species and the complex. The hydrodynamic radius can be used to infer the stoichiometry of the protein complex and the overall conformational arrangement in solution.

View application notes

Quantifying the stoichiometry of protein–protein interactions

Technique comparison

How does the Fluidity One-W compare to other techniques

There is a number of other techniques that are currently used to determine protein binding affinity, all with their own strengths and weaknesses, the Fluidity One-W is no different. We believe it should be a tool in your arsenal to compliment other methods, but you need to know how it compares to the other techniques.

View application notes

Affinity of PD-1/PD-L1 interaction—a comparison between SPR, MST, ITC and MDS

Trial the Fluidity One-W yourself

How does the Fluidity One-W work?

Sounds like this could support your research?

Get in touch with our scientists to discuss if our technology could work for you.

Take a closer look

Fluidity One-W highlights

In-solution, binding affinity measurement using microfluidic diffusional sizing (MDS)
Low sample requirement — Just 5μl per sample
High reproducibility and accuracy (even at low concentrations)
Easy-to-use interface and consumable management
Disposable single use chips and contained waste minimize risk of cross contamination between measurements and reduce cleaning and setup times

Fluidity One-W specifications

Sizing
- Range (hydrodynamic radius)
  
  0.7 – 20 nm
- Range (molecular weight)
  
  0.5 kDa – 14 MDa
- Accuracy
  
  ± 10%
- Precision
  
  CV < 10%
- Sensitivity
  
  1 nM Alexa Fluor™ 488
Operational Specifications
- Volume per measurement
  
  5 µL
- Run time
  
  Small proteins and peptides — 8 minutes
- Large proteins — 14 minutes
- Buffer compatibility
  
  Compatible with pure buffer or crude lysates
- Runs per reagent cartridge
  
  96
- Dimensions (D x W x H; cm)
  
  40 x 40 x 43
- Detection
  
  Fluorescence
- Label compatibility
  
  GFP, FITC, Alexa Fluor™ 488 and equivalents