Assess on-target protein interactions even in crude biological backgrounds
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The Fluidity One-W’s unique capabilities allow you to study protein complexes and their formation in crude biological backgrounds such as cell lysates or blood plasma. Absolute size measurements of protein complexes by the Fluidity One-W helps to confirm the identity of your complex and means you can control for off-target binding and false positive measurements. Combined with minimal sample preparation and automatic KD calculation, you can confidently analyse your protein at the earliest possible stage, reducing the time for important go/no-go decisions.
How does Fluidity One-W work?
The Fluidity One-W measures the rate of diffusion of proteins under steady state laminar flow in a microfluidic chip - a technique known as microfluidic diffusional sizing (MDS).
To do this, a stream of your fluorescently labelled protein is introduced alongside an auxiliary stream
These streams flow in parallel and because there is no convective mixing the only way your protein can migrate into the auxiliary stream is by diffusion, the rate of which will depend on the size of the protein. Small proteins will diffuse very rapidly, and large proteins and aggregates more slowly.
At the end the streams are re-split, and at this point the degree of diffusion is fixed. The quantity of protein in each stream is then determined by the fluorescence from the label. The ratio of the fluorescence between the two streams gives us the protein's hydrodynamic radius (Rh)
Fluidity One-W can measure proteins in buffer and in crude solutions like cell lysates or biological fluids, because only the labelled species is detected.
If we repeat the test using a mixture of labelled protein and unlabelled binding partner we can observe the degree of binding due to the change in average size. Only species including the labelled protein are detected and measured.
Titrating the binding partner against the labelled protein gives a binding curve and automatically generates a KD value. The hydrodynamic radius for the unbound protein and protein complex are also calculated.
With Fluidity One-W
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Assess binding in native conditions
By measuring your proteins and their interacting partners in a close-to-native, biological background, you can expect more transferable results that accurately represent natural biological processes.
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Study proteins in any solution
Work in crude biological mixtures with minimal preparation, while controlling for off-target binding. Gain valuable insights at the earliest possible stage.
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More for less
Gain meaningful insights into protein complexes and their formation with less sample and in less time.
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Look at the hard stuff
Solution state measurement coupled with high sensitivity means you can study proteins that are problematic for other systems such as:
-Membrane proteins
-Multi protein complexes
-Intrinsically disordered proteins -
Avoid wasted downstream processing
Assess the quality of your protein complex even in early stage fractions, allowing you to optimize protocols early. Use insights on protein size, state and yield to rationalize how you move forward before you begin time consuming purification.
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Pipette, plug, play
The minimal sample prep, easy-to-use design and wide dynamic range means you will spend less time setting up, and more time generating the data you need.
Would you like to be an early adopter?
The Fluidity One-W is currently in development, and we’re enrolling early access partners to help us define the specification of the instrument based on their applications. If you'd like to learn more and potentially participate then get in touch
Anticipated features
Monitor protein binding events entirely in solution, with confidence that you're on target, eliminating any artefacts and allowing you to study protein in near native state.
- In-solution, on target determination of complex formation in a single measurement using Microfluidic Diffusional Sizing (MDS)
- Low sample requirement – Just 5μl per sample
- High reproducibility and accuracy (even at low concentrations)
- Easy-to-use interface and consumable management
- Wide dynamic range allows you to determine nM to mM KDs
- Obtain an automatically calculated KD after a simple titration
- Disposable single use chips and contained waste minimize risk of cross contamination between measurements and reduce cleaning and setup times
- Sizing of any fluorescently labelled molecule - Proteins, lipids, carbohydrates, oligonucleotides, polymers or nanoparticles
- Broad buffer compatibility – suitable for use with any biologically compatible buffer, even those containing detergents
- Over the air software updates deliver ongoing system improvements
- Manufactured under ISO 9001 quality management system
*The Fluidity One-W is currently in development. Exact specifications are not yet confirmed and may be subject to change
Sounds like this could support your research?
Get in touch, our applications scientists will be happy to discuss your lab's protein research needs.
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