Fluidity One-W Serum
Scientists, clinicians and the biopharma industries across the world are trying to understand how proteins and their interactions effect sickness and health.
The ability to accurately assess the immune response is crucial to better understand infectious diseases like COVID‑19.
Traditional immunoassays such as ELISAs offer only an incomplete picture of the immune response against SARS‑CoV‑2 as they combine affinity and concentration into a single, less-precise measure known as titer.
Titers cannot distinguish between large numbers of weak-binding antibodies and small numbers of strong-binding antibodies.
Understanding antibody responses on the basis of quantitative fingerprints of affinity and concentration could have major implications for plasma transfer treatments, vaccine and therapeutic development, and the ongoing monitoring of immunity.
Characterization and quantification of antigen-antibody interactions directly in minimally diluted serum
Independent determination of antibody affinity and concentration for comprehensive immune response assessments in real time
Elimination of non-specific binding and other binding artifacts for accurate characterization of protein interactions
Determine antibody affinity (antibody binding strength) and concentration directly in patient serum for a comprehensive immune response assessment.
Characterize protein interactions in biological mixtures such as crude lysates, plasma without surface constraints or binding artifacts.
Our approach uses in-solution measurements to eliminate the many drawbacks of immobilized assays including non-specific binding and binding artifacts.
As a result, our assays can be run in native complex backgrounds like minimally diluted serum to better predict in-vivo performance.
Fluidity One-W Serum highlights
Range (hydrodynamic radius)
0.7 – 20 nm
Range (molecular weight)
0.5 kDa – 14 MDa
CV < 10%
1 nM Alexa Fluor™ 647
Volume per measurement
Small proteins and peptides — 8 minutes
Large proteins — 14 minutes
Compatible with pure buffer or crude lysates
Runs per reagent cartridge
Dimensions (D x W x H; cm)
40 x 40 x 43
Alexa Fluor™ 647 and equivalents