Fluidity One-W Serum

Quantify protein interactions in complex backgrounds

Fluidity One-W Serum instrument

Fluidity One systems offer a novel approach to determine affinity (KD), size, concentration, and stoichiometric information when a labeled protein interacts with DNA, lipids, antigens or other proteins

  • Characterize protein interactions in crude lysates, serum or plasma

    Eliminate risk of binding artefacts or other surface constraints by measuring protein interactions directly in solution

  • Determine KD and concentration simultaneously

    Measure antibody affinity profiles directly in minimally diluted serum or plasma

  • Comprehensively assess immune response

    Quantify immune responses to antigens
    Quantify viral neutralization capacity

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Measure directly in complex sample backgrounds

Antibody-affinity profiling in serum – SARS-COV-2 anti-spike S1 antibody against SARS-CoV-2 RBD (receptor binding domain)

  • Measure close to physiologically-relevant conditions
  • Reduce or eliminate time-consuming purification steps
  • Minimize risk of dilution effects
  • Minimize sample consumption – 60-100 μL* sample per KD

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antibody-affinity in serum - Fluidity One-W Serum (example 1)

Results independent of sample background

Antibody-affinity profiling – SARS-COV-2 anti-spike S1 antibody against SARS-CoV-2 RBD

  • Measurements in buffer or complex backgrounds, provide corresponding results
  • Quantitative analysis of protein–protein interactions (KD and binding-site concentrations)
  • Determine affinity, KD, and absolute concentration of antibody binding sites

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affinity profiling of sars-cov-2 antibody binding curve

Determine affinity and concentration with one technique

Immunoprofiling in serum during infection – Characterizing the immune response to viral infections

A collaboration with Prof. Adriano Aguzzi and Prof. Tuomas Knowles.

Universitats-Spital-Zurich-logo       University-of-Cambridge-logo
  • No correlation between affinity or concentration and disease severity – Hospitalized and non-hospitalized COVID-19 patients in same cluster
  • Affinity KD spans significantly wider range than concentration

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Determine affinity and concentration with one technique - Fluidity One-W Serum (example 3)

Image courtesy of Professor Adriano Aguzzi and Professor Tuomas Knowles

Take a closer look

Fluidity One-W Serum highlights

  • Generate data in close to physiologically-relevant conditions
  • Reduce or eliminate need for time-consuming purification
  • Determine KD and concentration directly in solution
  • Minimize sample consumption – typically 60 – 100 μL per KD
  • Reveal changes in molecular conformation

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Fluidic Analytics is changing how proteins are characterised

We're doing this with novel, cutting edge technology and a fantastic, committed team

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