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The Fluidity One-W system enables quantification and characterization of any protein interaction – in solution and even with challenging targets
Data in agreement with reported literature
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KD of aptamer-protein interactions
Changes in hydrodynamic radius in solution: Protein A:IgG interaction
Fluidity One-W highlights
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Application
Determine size, KD and stoichiometry in aqueous buffer systems
Technology
Microfluidic Diffusional Sizing (MDS)
Run time
Typically 8 – 14 min per datapoint
Size range: hydrodynamic radius, Rh
1 – 20 nm
Accuracy of size determination
± 10%
Reproducibility of size determination
CV < 10%
Working range molecular weight
1.4 kDa – 14 MDa
Sensitivity range (labeled HSA in PBS)
1 nM – 1 μM Alexa Fluor™ 488
Typical sample consumption to determine protein KD
60 – 100 μL
Sample volume per datapoint
5 μL
Compatibility
Compatible with aqueous buffer systems
Fluorescent labels
Alexa Fluor™ 488 and equivalents | GFP and FITC | Fluidiphore labeling kit (fluidiphore rapid amine 503)
Data export
USB Mass Storage Device | Fluidity Cloud
Exported data file formats
CSV and JSON formats
Data output from Fluidity Cloud
Result tables, binding curves and affinity (KD), size (Rh) of complex and labeled species
Consumables
Kits containing chips and cartridges sufficient for 96 or 288 datapoints
Temperature control
Ambient
Operating environment
5 °C to 40 °C
Power requirements
100 – 240 V AC, 50 – 60 Hz
Safety and EMC standards
Designed to comply with all relevant safety and EMC standards
Dimensions (D x W x H; mm)
400 x 400 x 430
Weight (kg)
15 kg
Download the Fluidity One-W specification sheet
even in complex backgrounds or with challenging targets