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Home > Menu of Standard Assays – Products > Fluidity One-W Serum
The Fluidity One-W Serum system enables quantification and characterization of any protein interaction – even in complex backgrounds and even with challenging targets
Fluidity One-W Serum highlights
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Application
Determine size, KD, concentration and stoichiometry in buffer systems or complex backgrounds such as serum or plasma
Technology
Microfluidic Diffusional Sizing (MDS)
Run time
Typically 8 – 14 min per datapoint
Size range: hydrodynamic radius, Rh
1 – 20 nm
Accuracy of size determination
± 10%
Reproducibility of size determination
CV < 10%
Working range molecular weight
1.4 kDa – 14 MDa
Sensitivity range (labeled HSA in PBS)
1 nM – 1 μM Alexa Fluor™ 647
Typical sample consumption to determine protein KD
60 – 100 μL
Sample volume per datapoint
5 μL
Compatibility
Compatible with crude, complex backgrounds such as serum or plasma Compatible with aqueous and biological buffers including components such as TRIS, HEPES, PBS, NaCl, KCl, TWEEN, DMSO and DMF.
Datapoints per run
1 datapoint per run
Fluorescent labels
Alexa Fluor™ 647 and equivalents | RFP and Cy5
Data export
USB Mass Storage Device | Fluidity Cloud
Exported data file formats
CSV, JSON formats
Data output from Fluidity Cloud
Result tables, binding curves and affinity (KD), size (Rh) of complex and labeled species
Consumables
Kits containing chips and cartridges sufficient for 96 or 288 datapoints
Temperature control
Ambient
Operating environment
5 °C to 40 °C
Power requirements
100 – 240 V AC, 50 – 60 Hz
Safety and EMC standards
Designed to comply with all relevant safety and EMC standards
Dimensions (D x W x H; mm)
400 x 400 x 430
Weight (kg)
15
Download the Fluidity One-W Serum specification sheet
even in complex backgrounds or with challenging targets