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The Fluidity One-M system enables quantification and characterization of any protein interaction – even in complex backgrounds or with challenging targets
Fluidity One-M highlights
Complete the form below to discuss how the Fluidity One-M system can provide simple solutions to all research problems.
Application
Determine KD, and concentration in biological buffer systems or backgrounds such as serum or plasma
Technology
Microfluidic Diffusional Sizing (MDS)
Run time
Typically within 25 min for 24 datapoints to determine KD
Size range: hydrodynamic radius, Rh
1 – 20 nm
Accuracy of size determination
± 10%
Reproducibility of size determination
CV < 10%
Working range molecular weight
1.4 kDa – 14 MDa
Sensitivity range (labeled HSA in PBS)
100 pM – 10 μM Alexa Fluor™ 488 | 100 pM – 10 μM Alexa Fluor™ 647
Typical sample consumption to determine protein KD
50 – 80 μL
Sample volume per datapoint
3.5 μL (application dependent)
Compatibility
Compatible with crude, complex backgrounds such as undiluted serum or plasma Compatible with aqueous and biological buffers including components such as TRIS, HEPES, PBS, NaCl, KCl, TWEEN, DMSO and DMF
Datapoints per run
Up to 24 datapoints per run
Fluorescent labels
Alexa Fluor™ 647 and equivalents, RFP/Cy5, Alexa Fluor™ 488 and equivalents, GFP/FITC, Fluidiphore labeling kit (fluidiphore rapid amine 503)
Data export
USB Mass Storage Device / Fluidity Cloud
Exported data file formats
CSV
Data output from Fluidity Cloud
Result tables, binding curves and affinity (KD), size (Rh) of complex and labeled species
Consumables
Kits sufficient for 192 datapoints
Temperature control
25 °C (actively controlled)
Operating environment
15 °C to 30 °C
Power requirements
100 – 240 V AC, 50 – 60 Hz
Safety and EMC standards
Designed to comply with all relevant safety and EMC standards
Dimensions (D x W x H; mm)
666 x 432 x 489 (Drawer Out)
Weight (kg)
35
Download the Fluidity One-M product brochure
even in complex backgrounds or with challenging targets