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Home > Menu of Standard Assays – Products > Fluidiphore Rapid Amine 503
The fluidiphore rapid protein labeling kit is an easy to use protein labeling kit that only needs 30 minutes incubation time and zero purification. The background effects from unbound dye are eliminated due to the absorbance wavelength shifting and an increase in quantum yield once the dye is conjugated to its binding partner, meaning no purification is required. Additionally unreacted dye is hydrolyzed during the labeling procedure.
The fluidiphore rapid amine 503 is an amine-reactive fluorescent label that once conjugated to its binding partner absorbs at 503 nm making it compatible with Fluidic Analytics’ fluidity One-W instrument.
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The fluidiphore rapid amine 503 requires minimal preperation and no purification steps making it ideal for rapid protein labeling. The diagram below shows how quick and easy it is to label your proteins. Watch the instruction video or read the instructions for further information.
Chemical properties
1 mg lyophilized blue solid which is soluble in DMF, methanol and acetonitrile. DMSO can also be used but is not recommended.
Molecular weight
393.29
Absorption
503 nm (conjugated), 612 nm (free)
Emission
600 nm (conjugated), 665 nm (free)
Ɛ L/(mol cm)
24,000 (conjugated), 60,000 (free)
Quantum Yield
~50% (conjugated), less than 1% (free)
Storage
The lyophilized dye should be stored at 4 ºC. The reconstituted dye should be stored at -20 ºC sealed in a bag with desiccant and allowed to reach room temperature before taken out of the bag.
Get in touch, our applications scientists will be happy to discuss your application requirements with you.
