In this webinar, we discuss the latest data from the literature and application studies where MDS is used to assess a variety of protein interactions – even of difficult targets.
To date, protein interactions with antibodies, lipids, proteins and more have been assessed by this method, with studies in both pure and complex backgrounds.
The high sensitivity and solution-based nature of the technique make it well-suited to challenging targets. Studies on intrinsically disordered proteins and fibrillar aggregates are shown and discussed.
This novel, in-solution approach reports binding affinity, KD, and stoichiometry of the binding interaction, but crucially also provides absolute size before and after binding. This provides a simple means to assess secondary events after binding (such as aggregation) and provides size information on the binding target.