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The chips are designed to house the microfluidic channels to ensure reliability and reproducibility of results.
The microfluidic chips for the fluidity One-W are sold as part of a consumable pack with reagent cartridges. These packs come in 2 sizes: Small – 4 chip boxes (total 96 chips) and one reagent cartridge. Medium – 12 chip boxes (total 288 chips) and 3 reagent cartridges.
Composition
Cyclic olefin copolymer
Coating
PEG-based, water insoluble non-covalent treatment
Unit size
24 chips per box; 96 chips per kit
Shelf life
1 year when stored as instructed
Fluidity One-W disposable microfluidic chips are bundled with sufficient reagents cartridges for the number of chips you order. These cartridges contain everything you need to perform Microfluidic Diffusional Sizing on the Fluidity One–W and Fluidity One-W Serum. For advice on changing your reagent cartridge on the Fluidity One-W system visit our FAQ.
Much like disposable cuvettes for DLS, we recommend that each chip is used just once. While the re-use of chips is not restricted, we can only vouch for the result obtained with the first use of each chip. This is because re-use increases the risk of introducing air into the system, as well as potential issues associated with protein adhesion and multiple actuations of the on-chip valve.
The microfluidic chips are made from injection molded COC — this allows excellent reproducibility between batches.
There may be a small amount of sample loss associated with “stickiness” of proteins, which is sample dependent. Our chips are coated to minimize protein adhesion and reduce this risk.
The layout of the disposable chips is fixed due do the prohibitive cost of a new injection mold tool.
We do have chip prototyping capabilities however, and we’re always open to discussing potentially novel and innovative applications of our technology. Please contact us if you have a specific requirement and we will be happy to discuss.
No. The flow rate is changed when the protein size is selected as you begin a measurement, but the auxiliary fluid is supplied with the reagent cartridge and remains the same.
If you would like to discuss custom reagent cartridges that include your auxiliary fluid of choice please contact us.
One cartridge contains enough reagents to run approximately 96 measurements which is equivilent to 4 chip boxes. The percentage remaining is indicated in the top right of the instrument display. Changing the cartridge takes a few minutes and does not require any special tools.
The exact protocol may vary depending on the software version of your instrument — check the user manual for full instructions on how to perform the change.
Reagent cartridges are disposable and should not be re-filled.
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