Introducing the

Fluidity One Platform

The Fluidity One platform harnesses microfluidic diffusional sizing (MDS) technology – a fundamentally new way of determining affinity (KD), size, concentration, and stoichiometric information when proteins interact with DNA, lipids, or other proteins.

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Quantify and characterize any protein interaction – in solution, even with challenging targets


Molecular size, KD, concentration and stoichiometry

Quantify and characterize any protein interaction – even in complex backgrounds, even with challenging targets

Molecular size, KD, concentration and stoichiometry

Quantify and characterize any protein interaction – even in complex backgrounds, even with challenging targets

Molecular size, KD, concentration and stoichiometry in a single experiment

Experience first-hand how Microfluidic Diffusional Sizing adds power to your protein interaction toolbox

Services | Assay Development | Solutions

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Find the instrument that’s best for you:

Application

Fluidity One-WFluidity One-W SerumFluidity One-M
TechnologyMicrofluidic Diffusional Sizing (MDS)Microfluidic Diffusional Sizing (MDS)Microfluidic Diffusional Sizing (MDS)
Determine molecular size
Determine affinity (KD)
Determine concentration
Determine stoichiometry
Determine KD, concentration, stoichiometry and molecular size in a single experiment
System - complex backgrounds e.g., cell lysates, serum and saliva

Interaction analysis

Fluidity One-WFluidity One-W SerumFluidity One-M
Run timeTypically 8 – 14 min per datapointTypically 8 – 14 min per datapointTypically under 25 min for 24 datapoints to determine KD
Datapoints per run1124
Working range molecular weight1.4 kDa – 14 MDa1.4 kDa – 14 MDa1.4 kDa – 14 MDa
Sensitivity range (labeled HSA in PBS) Alexa Fluor™ 4881 nM – 1 μM
100 pM – 10 μM
Sensitivity range (labeled HSA in PBS) Alexa Fluor™ 6471 nM – 1 μM100 pM – 10 μM
Typical sample consumption to determine protein KD60 – 100 μL60 – 100 μL50 – 80 μL

Compatibility

Fluidity One-WFluidity One-W SerumFluidity One-M
Compatible with aqueous buffer
Compatible with aqueous and biological buffers including components such as TRIS, HEPES, PBS, NaCl, KCl, TWEEN, DMSO and DMF
Compatible with crude, complex backgrounds such as serum or plasma

Fluorescent labels

Fluidity One-WFluidity One-W SerumFluidity One-M
Alexa Fluor™ 647 and equivalents
RFP/Cy5
Alexa Fluor™ 488 and equivalents
GFP/FITC
Fluidiphore labeling kit (fluidiphore rapid amine 503)

Specifications

Fluidity One-WFluidity One-W SerumFluidity One-M
Temperature controlAmbientAmbient25 °C (actively controlled)

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Download the Fluidity One systems comparison sheet

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The Fluidity One platform enables quantification and characterization of any protein interaction –

even in complex backgrounds or with challenging targets

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Learn more about the Fluidity One Platform

See MDS technology in action

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