Calculators page
Results
Volumes
Type of reagent to add to the mixture
Volume (µL)
| Buffer | 0 µL |
| Protein stock solution | 0 µL |
| Dye stock solution | 0 µL |
It is recommended to dilute the stock solution of dye so that the volume required is greater than 1 µL
Additional information
Amount of each reagent in the mixture
| DMSO in labeling miture | 0 % |
| Protein in labeling mixture | 0 nmol |
| Dye in labeling mixture | 0 nmol |
DMSO concentrations higher than 1.8% might interfere with the structural integrity of the protein. In case the concentration of DMSO in the labeling mixture exceeds 1.8%, the sheet will ask for the addition of buffer to reduce the DMSO concentration to 1.8%. For DMSO concentrations <1.8%, no additional volume of buffer is required and this field will show 0.0 µL.
In case either the volume of protein or the volume of dye to be added to the labeling mixture are <1.0 µL, it is recommended to dilute the respective stock solution.