Is my protein high quality?
Robust assessment of protein quality is critical in everything from routine protein analysis in basic and translational research through to the manufacture of protein based bio-pharmaceuticals.
Fluidic Analytics’ technology enables rapid analysis of protein quality using tiny amounts of material, providing valuable insights into oligomeric state, stability, aggregation and folding. Make measurements without calibration and without interference from detergents.
The quantity of protein in each stream is then determined using an amine reactive fluorogenic dye. The combined total fluorescence is used to determine the concentration of protein in solution, and the ratio of fluorescence between the two streams used to determine the diffusion co-efficient of the protein, which in turn is used to determine the protein’s hydrodynamic radius.
Because diffusion occurs while the protein is in its native state, the reported size is that of the native protein in solution. And because this process all takes place in microfluidic volumes, the measurement can be performed quickly and with minimal sample input.
Quality insights with less effort
Protein size can offer direct insights into degradation, oligomerization and aggregation. The Fluidity One combines label-free size measurement, high sensitivity detection and intuitive, plug and play design for rapid analysis of protein state at low concentrations, even in detergents.
Get more for less
Gain meaningful quality insights from hydrodynamic radius (Rh) and concentration in a single read, with less sample and in less time.
Look at the hard stuff too
Solution state measurement coupled with high sensitivity means you can study proteins that are problematic for other systems.
Pipette, plug, play
The minimal sample prep, easy-to-use design and wide dynamic range means you can skip lengthy setup and get to the results faster.
Let us do the work
Pre-configured just for proteins, with no calibration required, allowing you to focus on data generation rather than setup.
Intuitive design, straightforward consumable management and automated analysis means minimal training and no technical oversight required.
No IT department
Fully integrated user interface and over-the-air software updates mean no IT team needed to troubleshoot peripherals.
Ready for a demo?
Speak to a member of our team to discuss your applications for the Fluidity One and organize a demo.
From small peptides to large complexes
Using just 5 µL sample, Fluidity One can report hydrodynamic radius values for a range of different size proteins, giving equivalent values to DLS and TDA (Viscosizer).
See further details on the full application note here.
Accurate measurements at low concentrations
Even at low concentrations Fluidity One can provide reliable sizing even when other methods are no longer accurate.
See the full experimental data on our application note here.
Application noteProtein Size as an Indication of Structure
Molecular weight (Mw) is a commonly used, and for many scientists a readily understood, parameter to describe the size of a protein or complex. Here we show how hydrodynamic radius (Rh) can be used in combination with Mw to provide insights into the shape and structure of proteins and illustrate how Mw alone may not always provide a complete picture.
Application noteInterleukin-2 stability in changing buffer and temperature conditions
The stability of interleukin-2 in different buffers and storage temperatures is evaluated using the Fluidity One. We find that IL-2 forms aggregates within 24 hours in some buffers, and that the Fluidity One provides a simple means to evaluate the stability of proteins across different conditions.
- In-solution determination of size and concentration in a single measurement using Microfluidic Diffusional Sizing (MDS)
- Label free measurement
- Minimal setup as no calibration required
- Low sample requirement – use as little as 50 ng per measurement
- High reproducibility and accuracy (even at low concentrations)
- Easy-to-use interface and consumable management
- Disposable single use chips and contained waste minimise risk of cross contamination between measurements and minimize cleaning and setup times
- Over the air software updates deliver ongoing system improvements
- Manufactured under ISO 9001 quality management system
- Easy to troubleshoot results as grounded in well understood scientific principles