Applications

A growing range of applications using Microfluidic Diffusional Sizing

Click on one of the applications below to learn how Fluidic Analytics technology is being applied to generate valuable insights in these areas, or scroll down to see our complete list of application notes.

Application Notes

  • Application note

    Quantitation of low Tryptophan and Tyrosine peptides using the Fluidity One

    UV absorbance is commonly used as a fast method for protein and peptide quantitation, however samples must contain tryptophan (Trp) or tyrosine (Tyr) residues to be detected. Here we demonstrate the ability of the Fluidity One to assess the concentration of a range of samples which have low or no Trp and Tyr content.

  • Application note

    Protein Size as an Indication of Structure

    Molecular weight (Mw) is a commonly used, and for many scientists a readily understood, parameter to describe the size of a protein or complex. Here we show how hydrodynamic radius (Rh) can be used in combination with Mw to provide insights into the shape and structure of proteins and illustrate how Mw alone may not always provide a complete picture.

  • Application note

    Oligomerization of Interleukin-2

    A commercially available human interleukin-2 is assessed by microfluidic diffusional sizing on the Fluidity One across a dilution series. The hydrodynamic radius is observed to increase with increasing concentration, in a way which suggests a monomer-trimer equilibrium with positive cooperativity is established.

  • Application note

    Interleukin-2 stability in changing buffer and temperature conditions

    The stability of interleukin-2 in different buffers and storage temperatures is evaluated using the Fluidity One. We find that IL-2 forms aggregates within 24 hours in some buffers, and that the Fluidity One provides a simple means to evaluate the stability of proteins across different conditions.

  • Application note

    A comparison of Microfluidic Diffusional Sizing with Dynamic Light Scattering and Taylor Dispersion Analysis

    The established technologies of Dynamic Light Scattering (DLS) and Taylor Dispersion Analysis (TDA) are compared to Microfluidic Diffusional Sizing (MDS) for sizing proteins of varying molecular weights and at varying concentrations. We show that MDS offers comparable sizing of proteins over a range of sizes, and can provide consistent sizing to lower concentrations than the other techniques.

  • Application note

    Detecting insulin oligomerization using microfluidic diffusional sizing

    Insulin monomers self-assemble into hexamers, which is known to affect its level of uptake in the human body. Here we show that Microfluidic Diffusional Sizing (MDS) can be used to detect these changes.

Fluidity One applications

The speed, convenience and low sample consumption of the Fluidity One lends it to a number of routine lab applications

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