• Applications
  • Determining the size of biomolecules for the purpose of quality control, confirmation of complex formation, or detection of degradation and aggregation usually requires large amounts of sample, operates over limited concentration ranges, and does not work in complex backgrounds such as cell lysates. MDS offers a game-changing approach by monitoring the size of individual proteins or their complexes in buffer or crude matrices with minimal time and sample investment. 
    Overview

    Size measurements offer insights into protein quality and oligomeric states, important features during the drug development and formulation process. MDS measures size and goes beyond traditional methods to accelerate efficient drug development by requiring minimal sample volume and measurement time. This enables researchers to simplify their workflow to understand long-term stability, such as susceptibility to degradation or aggregation of a protein or protein-complex in buffer as well as in complex backgrounds such as lysate or serum. In addition, MDS tolerates high protein concentrations required for testing, for example, low-affinity complex formation prior to cryo-EM. 

    Obtain equivalent size measurements in complex backgrounds.
    Buffer
    Human Serum
    Figure 1. Microfluidic Diffusional Sizing assessment of the interaction of SARS-CoV-2 anti-spike S1 antibody against SARS-CoV-2 RBD in buffer (PBS-T) and > 90% serum matrix demonstrates equivalent affinities (boxed) and sizes of both the free RBD and RBD in-complex with target (circled).
    Case Study
    Engineered repeat proteins as scaffolds to assemble multi-enzyme systems for efficient cell-free biosynthesis.
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    To obtain insights into protein quality and oligomeric states we recommend the following: 

    Workflow specification and benefits:
    • 25 min run time for 24 measurements 
    • KD range from pM to µ 
    • Provides stoichiometry 
    • Amount of antibody 1-10 µg depending on affinity  
    • Amount of oligomer or fibril 10-25 µg 
    • 12 µL of sample per triplicate  
    • Use of complex background e.g. serum, lysate, CSF 
    • Quick & easy to perform 

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