Fluidity One-W Serum

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Fluidity One-W Serum

Quantify and characterize any protein interaction


The Fluidity One-W Serum system enables quantification and characterization of any protein interaction – even in complex backgrounds and even with challenging targets


Explore interactions in solution under physiologically-relevant conditions

  • Analyze samples in different backgrounds – from simple buffers to cell lysates, serum and saliva
  • Determine size, KD, concentration and stoichiometry in solution
  • Obtain simultaneous measurements of KD and concentration
  • Gain insights into interaction stoichiometry; distinguish on-target binding from off-target binding

 

Characterize polyclonal antibodies

Track functional immune response
in serum samples

Explore aggregation effects and distinguish between specific and
non-specific binding

Reveal therapeutic antibody / protein interaction mechanisms

Characterize disordered proteins or higher-order complexes under close to
in vivo conditions


Take a closer look

Fluidity One-W Serum highlights

  • Uses microfluidic diffusional sizing (MDS) technology to measure changes in molecular size (hydrodynamic radius) as binding events occur
  • Enables development of customized protocols to study a wide range of interactions – typical run time 8-14 minutes per datapoint
  • Eliminates risk of binding artefacts or other surface constraints – measure directly in solution – no surface immobilization
  • Minimizes consumption of precious samples – 5 μL per datapoint, 60-100 μL to determine KD

Register your interest

Complete the form below to register your interest in the Fluidity One platform.

Fluidity One-W Serum specifications

  • System

    • Application

      Determine size, KD, concentration and stoichiometry in buffer systems or complex backgrounds such as serum or plasma

    • Technology

      Microfluidic Diffusional Sizing (MDS)

    Interaction analysis

    • Run time

      Typically 8 – 14 min per datapoint

    • Size range: hydrodynamic radius, Rh

      1 – 20 nm

    • Accuracy of size determination

      ± 10%

    • Reproducibility of size determination

      CV < 10%

    • Working range molecular weight

      1.4 kDa – 14 MDa

    • Sensitivity range (labeled HSA in PBS)

      1 nM – 1 μM Alexa Fluor™ 647

    • Typical sample consumption to determine protein KD

      60 – 100 μL

    • Sample volume per datapoint

      5 μL

    • Compatibility

      Compatible with crude, complex backgrounds such as serum or plasma
      Compatible with aqueous and biological buffers including components such as TRIS, HEPES, PBS, NaCl, KCl, TWEEN, DMSO and DMF.

    • Datapoints per run

      1 datapoint per run

    • Fluorescent labels

      Alexa Fluor™ 647 and equivalents | RFP and Cy5

    • Data export

      USB Mass Storage Device | Fluidity Cloud

    • Exported data file formats

      CSV, JSON formats

    • Data output from Fluidity Cloud

      Result tables, binding curves and affinity (KD), size (Rh) of complex and labeled species

    Consumables

    • Consumables

      Kits containing chips and cartridges sufficient for 96 or 288 datapoints

    Specifications

    • Temperature control

      Ambient

    • Operating environment

      5 °C to 40 °C

    • Power requirements

      100 – 240 V AC, 50 – 60 Hz

    • Safety and EMC standards

      Designed to comply with all relevant safety and EMC standards

    Dimensions

    • Dimensions (D x W x H; mm)

      400 x 400 x 430

    • Weight (kg)

      15

Download the Fluidity One-W Serum specification sheet

The Fluidity One platform enables quantification and characterization of any protein interaction –

even in complex backgrounds or with challenging targets

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